Abstract: Objective: This preliminary study aimed to explore the collagen types of engineered cartilage formed from co-culturing costal chondrocytes (CCs) and dental pulp cells (DPCs). Methods: Human CCs and human DPCs were mixed at a ratio of 1∶1 to form pellets, and were co-cultured in chondrogenic medium to promote chondrogenic differentiation of DPCs. Then the picric acid / sirius red staining and polarization microscopy were  used to explore the collagen types of the engineered cartilage. Results: The formed engineered cartilage was mainly composed of type I collagen, simultaneously including type II and type III collagen. The histological structure of engineered cartilage was similar to that of costal cartilage, temporomandibular joint disc cartilage, and the proliferation layer of condylar cartilage. Conclusion: The engineered cartilage formed from co-culturing CCs and DPCs was similar with fibrous cartilage.

Key words: dental pulp cells, costal chondrocytes, co-culturing, engineered cartilage, type

摘要： 目的： 研究牙髓细胞和肋软骨细胞共培养形成的组织工程软骨的胶原类型。方法：人肋软骨细胞和牙髓细胞按1∶1的比例用微团法共培养来促进其向软骨细胞分化。应用苦味酸天狼猩红染色，偏正光检测法研究形成的组织工程软骨胶原类型，并与颅颌面区域软骨进行比较。结果：肋软骨细胞和牙髓细胞微团法共培养形成的组织工程软骨中以I型胶原为主，同时包括II和III 型胶原纤维，其纤维结构组成与肋软骨、髁突软骨增殖层及颞下颌关节盘的纤维结构相似。结论：肋软骨细胞和牙髓细胞共培养可形成的组织工程软骨，其组织结构与纤维软骨相似。

关键词: 牙髓细胞,  ,  , 肋软骨细胞,  ,  , 共培养,  ,  , 组织工程软骨,  ,  , 类型

CLC Number: 

• R782.2