《Journal of Oral and Maxillofacial Surgery》 ›› 2016, Vol. 26 ›› Issue (6): 385-. doi: 10.3969/j.issn.1005-4979.2016.06.002

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Drug-loaded Nanoparticles within Gelatin Scaffolds Inhibits MMPs Expression in Periodontal Ligament Cells

WANG Ya-bing, SU Jian-sheng   

  1. Department of Prosthodontics, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration
  • Received:2016-02-17 Revised:2016-06-22 Online:2016-12-28 Published:2017-06-30

载药纳米粒明胶纤维缓释支架对牙周膜细胞MMPs的影响

王雅冰,苏俭生   

  1. 同济大学口腔医学院·同济大学附属口腔医院口腔修复教研室,上海牙组织修复与再生工程技术研究中心
  • 通讯作者: 苏俭生,教授. E-mail: sjs@tongji.edu.cn E-mail:sjs@tongji.edu.cn
  • 作者简介:王雅冰(1989—),男,上海人,硕士研究生. E-mail: 13120745599@163.com
  • 基金资助:

    国家自然科学基金(81371949、81572114),上海市科学技术委员会项目(13411951201)

Abstract: Objective: The study aimed to evaluate whether the SB203580-loaded nanoparticles-gelatin (Ms-SB203580-gelatin) scaffolds are related to reduction of  matrix metalloproteninases(MMPs) expression in human periodontal ligament cells (hPDLCs). Methods: The drug-loaded micelles(Micelles-SB203580, Ms-SB203580)was obtained by dialysis bag method and fabricate drug-loaded gelatin scaffolds by electrospining. Cell compatibility and the gene as well as protein expression of  MMP-2、MMP-13 were measured. Results: The gelatin scaffolds contain drug-loaded micelles were proved by TEM,  The hPDLCs seeded at the scaffolds demonstrated a good biocompatibility. MMP-2 and MMP-13 expression were stain slightly in the experimental group  than that of the positive group. Conclusion: The results suggest that Ms-SB203580-gelatin scaffolds have a potential to obviously reduce  inflammation and may be effective for alveolar bone repair in the future.

Key words:  immunosuppressor, scaffolds, human periodontal ligament cells, MMPs

摘要: 目的:研究载免疫抑制剂SB203580的纳米粒-明胶(Ms-SB203580-gelatin)纤维缓释支架对牙周膜细胞(human periodontal ligament cells,hPDLCs)的基质金属蛋白酶(matrix metalloproteninases,MMPs)表达的影响。方法:通过透析法制备载药纳米胶束(Micelles-SB203580,Ms-SB203580),并通过电纺法构建载纳米粒明胶支架,检测支架材料的细胞相容性和对牙周膜细胞MMP-2、MMP-13基因和蛋白的表达影响。结果:透射电镜显示载药纳米粒能负载于明胶纤维中;细胞与支架复合后增殖情况良好;实验组MMP-2、MMP-13的表达低于阳性对照组。结论:载药纳米粒明胶纤维缓释支架能明显地抑制炎症因子,可进一步应用于牙槽骨骨组织工程。

关键词: 免疫抑制剂, 支架, 牙周膜细胞, MMPs

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