《Journal of Oral and Maxillofacial Surgery》 ›› 2020, Vol. 30 ›› Issue (5): 288-294. doi: 10.3969/j.issn.1005-4979.2020.05.005

• Basic Scientific Study • Previous Articles     Next Articles

A Preliminary Study on the Role of JAK2/STAT3 Signaling Pathway in Estrogen-related BMSCs Senescence

WU Wenjing(), FU Jiayao, WEI Yu, MA Pengfei, WU Junhua()   

  1. Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Prosthodontics, School and Hospital of Stomatology, Tongji University, Shanghai 200072, China
  • Received:2019-12-12 Revised:2020-08-15 Online:2020-10-28 Published:2020-10-27

JAK2/STAT3通路在雌激素缺乏相关骨髓间充质干细胞衰老中的作用初探

武文婧(), 傅稼耀, 魏玉, 马鹏飞, 吴珺华()   

  1. 上海牙组织修复与再生工程技术研究中心,同济大学口腔医学院,同济大学附属口腔医院口腔修复科,上海 200072
  • 通讯作者: 吴珺华,副教授. E-mail: wujunhua_sh@tongji.edu.cn
  • 作者简介:

    武文婧(1993—),女,辽宁朝阳人,硕士研究生. E-mail:

  • 基金资助:
    上海市自然科学基金项目(19ZR1462000)

Abstract:

Objective: To investigate the role of janus kinase 2/signal transducer and activator of transcription 3 (JAK2/STAT3) signaling pathway in bone marrow mesenchymal stem cells(BMSCs) senescence caused by estrogen deficiency. Methods: BMSCs of mice were extracted and treated with H2O2 in appropriate concentration to be senescent. Estrogen analog 17β-estradiol(E2) or JAK pathway inhibitor (ruxolitinib) was further added, and senescence indicators and changes of JAK2/STAT3 signaling pathway were detected by quantitative polymerase chain reaction(qPCR), Western blot and SA-β-gal staining. Results: The senescence of BMSCs induced by H2O2 can be inhibited by estrogen and accompanied with the down-regulation of JAK2/STAT3 signaling. Further suppression of JAK2/STAT3 signaling pathway can also rescue BMSCs senescence. Conclusion: The JAK2/STAT3 signaling pathway is a target of estrogen-related BMSCs senescence, and inhibition of JAK2/STAT3 can rescue BMSCs senescence.

Key words: cellular senescence, janus kinase 2/signal transducer and activator of transcription 3(JAK2/STAT3), bone marrow mesenchymal stem cells (BMSCs), estrogen

摘要:

目的: 探究非受体型蛋白酪氨酸激酶2/信号转导与转录激活子3(janus kinase 2/signal transducer and activator of transcription 3, JAK2/STAT3)信号通路在雌激素缺乏所致骨髓间充质干细胞(bone marrow mesenchymal stem cells, BMSCs)衰老中的作用。方法: 提取小鼠BMSCs,并用适宜浓度的过氧化氢(H2O2)诱导其衰老。进一步添加雌激素类似物17β-雌二醇(17β-estradiol,E2)或JAK通路抑制剂(鲁索替尼),用实时荧光定量聚合酶链反应(quantitative polymerase chain reaction, qPCR)、Western印迹法(Western blot)、β-半乳糖苷酶(SA-β-Gal)染色法检测其衰老指标及JAK2/STAT3信号通路的改变。结果: H2O2诱导的BMSCs衰老可被雌激素抑制,且伴随着JAK2/STAT3信号的下调,而进一步抑制JAK2/STAT3信号通路也可以挽救BMSCs的衰老。结论: JAK2/STAT3信号通路是雌激素调控BMSCs的衰老靶点,且抑制该通路可以挽救BMSCs的衰老。

关键词: 细胞衰老, 酪氨酸激酶2/信号转导与转录激活子3, 骨髓间充质干细胞, 雌激素

CLC Number: