Abstract:  Objective: To evaluate the osteogenic activities of Bio-Oss after combining with fibrin glue（FG） and ADM in reconstruction of canine mandibular defects． Methods: The second and fourth premolar teeth and the second molar teeth were extracted bilaterally，in 9 hibrid canines，resulting in 6 bone defects(1 cm × 1 cm)in each canine． Bio-Oss ， Bio-Oss+FG ，Bio-Oss+FG+ADM were implanted into the bone defects randomly． Three Canines in every group were executed after 4，8，and 12 weeks to observe the healing of soft and hard tissues．The property of Bio-Oss and ADM were observed via gross specimen，the morphology of the newly-formed bone and ADM was observed by tissue sections．The proportion of newly-formed bone was obtained by computer image analysis(SAS software，analysis of variance)．Results: The soft tissue all coalesced. The patch was existed after 12 weeks. The rate of new bone in ADM group was higher than that in Bio-Oss group.  Conclusion:  The tissue patch can guide the bone regeneration, and thick the soft tissue. It can improve the osteogenic activities of Bio-Oss after combining with fibrin glue.

Key words: tissue patch, Bio-Oss, mandible bone defect, fibrin glue, shaping

摘要： 目的：分析口腔组织补片（ADM）是否可以改善Bio-Oss经纤维蛋白胶（FG）塑形后诱导成骨性能下降的问题。方法：9条杂种犬，每只拔除双侧下颌第2、4前臼齿和第2臼齿，造成6个1cm×1cm大小的骨缺损区，将Bio-Oss+FG+ADM、Bio-Oss+FG、Bio-Oss共3组材料随机植入骨缺损区，每组2处缺损。分别在术后4、8、12周各处死3条实验犬，观察软硬组织的愈合情况，大体标本观察补片生长情况，组织切片观察新生骨组织形态、组织补片的愈合情况，计算机图像分析得出新生骨百分率，利用SAS软件进行方差分析。结果：软组织均一期愈合；组织补片在12周时仍未吸收，紧密结合在骨组织表面，显微镜下显示补片与新生骨组织紧密结合。实验组新生骨百分比在4、8、12周均高于对照组。结论：组织补片吸收时间超过12周，可以促进经纤维蛋白胶塑形后的Bio-Oss成骨性能，同时可以促进覆盖软组织在植入物表面的早期附着，对防止伤口裂开有一定预防作用。

关键词:  , 组织补片；无机牛骨；下颌骨缺损；纤维蛋白胶；塑形

CLC Number: 

• R782.23