《Journal of Oral and Maxillofacial Surgery》 ›› 2015, Vol. 25 ›› Issue (1): 34-. doi: 10.3969/j.issn.1005-4979.2015.01.006

• Basic Scientific Study • Previous Articles     Next Articles

Rapid Prototyping of Nano-HA/PCL Composite Scaffolds and Osteogenic Potential of Bone Marrow Stroma Cells of Canine in vitro

GUO Ling-yun, ZHANG Jin-e, YUAN Jian-bing, HUANG Yuan-liang   

  1. 1. Department of Stomatology, Shanghai Municipal Oriental Hospital, Shanghai 200120; 2. School of Mechanical Engineering, Shanghai Jiaotong University, Shanghai 200030, China
  • Online:2015-02-28 Published:2015-04-29

快速原型技术制备Nano-HA/PCL支架与犬骨髓基质干细胞体外复合研究

郭凌云,张劲娥,袁建兵,黄远亮   

  1. 1. 上海市东方医院口腔科,上海   200120;2. 上海交通大学机械与动力工程学院,上海   200030
  • 通讯作者: 黄远亮,教授. E-mail:YLHuang0115@163.com
  • 作者简介:郭凌云(1977—),女,硕士,山西人,主治医师.
  • 基金资助:

    上海市浦东新区卫生局优青培养计划  (PWRq2011-03)

Abstract: Objective: To fabricate nano-hydroxyapatite/Poly-ε-caprolactone(nano-HA/PCL) scaffold with three-dimensional simulation of the root shape by using a rapid prototyping (RP) process. To evaluate biocompatibility of the scaffold used for bone tissue engineering. Methods:  Parietal bone image information of canine was obtained by CT scan, three-dimensional reconstruction images were performed by CAD, roots shape nano-HA/PCL scaffolds was obtained by RP technology. Canine 3rd passage bone marrow stromal cells (BMSCs) were seeded in nano-HA/PCL scaffolds and grown in osteogenic medium. Cells were harvested after 1, 3, and 7 days. BMSCs proliferation and ALP activity were measured. Results: Scanning electron micrographs showed the BMSCs adhered to the inner surface of the scaffolds. Cell proliferation steadily increased with time, and alkaline phosphatase activity in the scaffolds at 7- day and 14- day were enhanced. Conclusion: The osteogenic potential of BMSCs seeded at the rapid prototyped nano-HA/PCL scaffolds demonstrate a good biocompatibility, and may be effective for animal experiment in alveolar bone repair in the near future.

Key words: rapid-prototyping technique, nano-hydroxyapatite/poly-ε-caprolactone(nano-HA/PCL)scaffold, bone marrow cells, biocompatibility, dogs

摘要: 目的:探讨快速原型制作纳米-羟基磷灰石/聚己内酯(nano-HA/PCL)根形三维支架的细胞生物相容性,为进一步体内实验提供研究基础。方法:通过CT 扫描获得犬头颅骨影像信息,以CAD 软件实现犬下颌牙根形态的三维重建影像,应用快速原型(RP)技术制作nano-HA/PCL根形支架,经犬骨髓基质细胞接种后体外复合培养,检测支架材料的细胞相容性。结果:通过CT影像和快速原型技术,可实现解剖根形态结构nano-HA/PCL三维仿真支架。细胞支架复合培养后,扫描电镜显示细胞生长附着于支架表面;细胞与支架复合后增殖情况良好;7 d和14 d碱性磷酸酶活性高于对照组。结论:体外复合培养结果表明,RP技术制备的nano-HA/PCL支架和骨髓基质细胞生物相容性较好,可进一步应用于牙槽骨缺损骨组织工程修复动物实验。

关键词: 快速原形技术, 羟基磷灰石/聚己内酯支架, 骨髓基质细胞, 生物相容性,

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