《Journal of Oral and Maxillofacial Surgery》 ›› 2015, Vol. 25 ›› Issue (5): 326-. doi: 10.3969/j.issn.1005-4979.2015.05.003

• Basic Scientific Study • Previous Articles     Next Articles

Curcumin Inhibits Human Oral Cancer Cell Growth by Suppressing EGFR Phosphorylation

LI Kui-fang1, FAN De-sheng2, LIU Ye1, FAN De-quan3   

  1. 1. Department of Pathology, Jiangyin Municipal Traditional Chinese Medicine Hospital, Jiangyin 214400, Jiangsu Province; 2. Department of Pathology, Shanghai Tongji Hospital, Tongji University, Shanghai 200065; 3. Department of Stomatology, Four Town Community Health Service Centre, Fengxian District, Shanghai 201421, China
  • Online:2015-10-28 Published:2015-12-17

姜黄素对口腔鳞癌SCC-25细胞EGFR信号通路表达的影响

李葵芳1,范德生2,刘晔1,范得泉3   

  1. 1. 江苏省江阴市中医院病理科,江苏   214400;2. 同济大学附属上海市同济医院病理科,上海   200065;3. 上海市奉贤区四团镇社区卫生服务中心口腔科,上海   201412
  • 通讯作者: 范德生,副主任医师. E-mail:bingliyisheng@126.com
  • 作者简介:李葵芳(1976—),女,江苏江阴人,主治医师,学士. E-mail:13306162658@189.cn

Abstract: Objective: To study whether Curcumin was associated with the signaling pathway expression of EGFR of human oral squamous cell carcinoma (OSCC) SCC-25 cells and its molecular mechanisms. Methods: The efficacy of curcumin on proliferation in SCC-25 cell line were investigated. The inhibition effect of curcumin on EGF-induced SCC-25 cells invasion were explored by transwell assay. The efficacy of curcumin on the activition of EGFR and its downstream signaling molecules Akt, ERK1/2 and STAT3 were investigated by western blot. Results: Curcumin inhibited SCC-25 cells proliferation and EGF-triggered SCC-25 cells invasion. Curcumin regulated the p-EGFR and EGFR downstream signaling molecules including Akt, ERK1/2 and STAT3. Conclusion: Curcumin reduced SCC-25 cells proliferation and invasion through inhibiting the phosphorylation of EGFR and EGFR downstream signaling molecules Akt, ERK1/2 and STAT3.

Key words: EGFR signaling pathways, curcumin, oral squamous cell carcinoma, target therapy

摘要: 目的:探讨姜黄素对口腔鳞癌SCC-25细胞EGFR信号通路表达的影响及其相关的分子机制。方法:不同浓度姜黄素作用细胞24 h,MTT法检测姜黄素对细胞增殖作用的影响,Transwell实验检测EGF 和姜黄素共同作用下细胞侵袭能力的变化,Western blot检测姜黄素对SCC-25细胞EGFR活性及下游信号分子(Akt、ERK1/2 和 STAT3 )表达的影响。结果:不同浓度姜黄素作用SCC-25细胞24 h后,均可抑制SCC-25细胞增殖,亦可以抑制EGF诱导的细胞侵袭转移。Western blot结果显示姜黄素对SCC-25细胞总EGFR表达无影响,但可以剂量依赖性抑制SCC-25细胞p-EGFR表达,同时可以剂量依赖性抑制EGFR信号通路下游转录因子Akt、ERK1/2 和 STAT3磷酸化。结论:姜黄素可剂量依赖性抑制SCC-25细胞EGFR磷酸化活性及其下游信号通路分子(Akt、ERK1/2、STAT3)的磷酸化。

关键词: EGFR信号通路;  , 姜黄素;  , 口腔鳞癌;  , 靶向治疗

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