Journal of Oral and Maxillofacial Surgery

MicroRNA-143-3p targeting RANK regulates the apoptosis and differentiation of dental pulp stem cells

LI Mingwei, WANG Yunxia, WANG Jing

《Journal of Oral and Maxillofacial Surgery》, 2022, 32(4): 209-216. DOI: 10.3969/j.issn.1005-4979.2022.04.002

基因	正向引物（5′-3′）	反向引物（5′-3′）
miR-143-3p	ACACTCCAGCTGGGGGTGAGAGATGAAGCACTG	TGGTGTCGTGGAGTCG
U6	CTCGCTTCGGCAGCACA	AACGCTTCACGAATTTGCGT
OPG	TCAATGGCTACACAGGACCA	CACTGTCACCTGGAAGCAGA
RANKL	CAGCCTTTTGCTCATCTCACTATTAA	GGTACCAAGAGGACAGACTCACTTTAA
RANK	TCCAACCTACGTGAAGGGATAC	GTGCCTGGTCTTGGCTTCTC
Runx2	CCGCCTCAGTGATTTAGGGC	GGGTCTGTAATCTGACTCTGTCC
OCN	AGGGCAGCGAGGTAGTGAA	TCCTGAAAGCCGATGTGGT
ALP	CAGAAGAAGGACAAACTGGG	ATTGTATGTCTTGGACAGAGC
BMP2	ATGTTCGCCTGAAACAGAGACCCA	CTTACAGCTGGACTTAAGGCGTTTC
GAPDH	TGCTTCACCACCTTCTTGA	TCACCATCTTCCAGGAGC

Table 1 Primer sequences

Other figure/table from this article

Figure 1 The effect of miR-143-3p expression level changes on the RANK mRNA expression level
Figure 2 Comparison of cell apoptosis rate in each group
Figure 3 Results of cell apoptosis detection
Figure 4 Alizarin red staining results (×200)
Figure 5 Comparison of absorbance values of alizarin red staining
Figure 6 The effect of miR-143-3p on the expression of genes in hDPSCs
Figure 7 The effect of miR-143-3p on the expression of proteins in hDPSCs
Figure 8 Potential binding sites (A) and luciferase activity (B) of miR-143-3p and RANK 3′ UTR