探讨口腔颌面部恶性肿瘤生物样本库的建设模式及其在临床预后研究中的应用价值，并分析影响患者预后的危险因素。

总结南京市口腔医院口腔颌面部恶性肿瘤生物样本库的建设及运行情况。基于该样本库，回顾性分析2013年1月—2018年12月于本院接受手术治疗的1 138例口腔颌面部恶性肿瘤患者的临床资料，建立临床病理数据库并随访。采用Kaplan-Meier法计算总体生存率，Log-rank检验进行单因素分析，Cox比例风险回归模型进行多因素分析。

本中心建立了集样本采集、处理、保存、质量控制及临床随访于一体的标准化口腔颌面部恶性肿瘤生物样本库；截至2023年12月，样本库累计纳入患者2 594例。本研究共纳入1 138例患者，平均随访时间为（45.37±21.16）个月，患者总体1、3、5年生存率分别为89.67%、80.02%和76.04%。多因素分析结果显示，年龄≥60岁[风险比（hazard ratio，HR）=1.439，95%置信区间（confidence interval，CI）：1.108~1.868]、肿瘤复发（HR=2.679，95%CI：1.962~3.657）及淋巴结转移（HR=3.312，95%CI：2.409~4.554）是影响患者总体生存率的独立危险因素（P<0.01）。

本中心建立的标准化口腔颌面部恶性肿瘤生物样本库可为相关临床与转化研究提供重要平台支持。基于该样本库的临床预后分析显示，年龄≥60岁、肿瘤复发及淋巴结转移是影响患者预后的独立危险因素，对患者风险分层及个体化治疗具有重要指导意义。

To explore the construction model of a biobank for oral and maxillofacial malignant tumors and its application value in clinical prognosis research, and to analyze the risk factors influencing patient prognosis.

The establishment and operation of the biobank for oral and maxillofacial malignant tumors at Nanjing Stomatological Hospital were summarized. Based on this biobank, clinical data of 1 138 patients with oral and maxillofacial malignant tumors who underwent surgical treatment at the hospital from January 2013 to December 2018 were retrospectively analyzed. A clinicopathological database was established and follow-up was conducted. Overall survival rate was calculated using the Kaplan-Meier method, univariate analysis was performed using the Log-rank test, and multivariate analysis was performed using the Cox proportional hazards regression model.

A standardized biobank for oral and maxillofacial malignant tumors integrating sample collection, processing, storage, quality control, and clinical follow-up was established at our center. As of December 2023, the biobank had enrolled a total of 2 594 patients. A total of 1 138 patients were included in this study, with a mean follow-up time of 45.37±21.16 months. The overall 1-, 3-, and 5-year survival rates were 89.67%, 80.02%, and 76.04%, respectively. Multivariate analysis showed that age ≥60 years [hazard ratio (HR)=1.439, 95% confidence interval (CI): 1.108–1.868], tumor recurrence (HR=2.679, 95%CI: 1.962–3.657), and lymph node metastasis (HR=3.312, 95%CI: 2.409–4.554) were independent risk factors for overall survival (P<0.01).

The standardized biobank for oral and maxillofacial malignant tumors established at our center provides important platform support for relevant clinical and translational research. Clinical prognostic analysis based on this biobank shows that age ≥60 years, tumor recurrence, and lymph node metastasis are independent risk factors for patient prognosis, which has significant guiding value for risk stratification and individualized treatment.