摘要： 目的：探究调控腮腺分泌的中枢神经系统内相关核团的分布。方法：选取8~12周龄C57BL/6小鼠共3只，在小鼠腮腺分3个位点注射伪狂犬病毒重组体（PRV） 3μL，注射病毒5 d后荧光显微镜下观察小鼠大脑中被标记的核团分布。结果：PRV感染5 d后，被标记的脑区在后脑主要包括下泌涎核、后脑区的网状结构、孤束核、三叉神经脊束核、面神经核、中缝核群、蓝斑、蓝斑下核、三叉神经上核、臂旁核等；中脑及间脑主要有中脑导水管周围灰质、中脑区的网状结构、底丘脑旁核、未定带、下丘脑外侧区、下丘脑室旁核尾部、视前内侧区等；端脑中有中央杏仁核、终纹床核、外侧隔核、背外侧内嗅皮层、颗粒状岛叶皮层、异粒状岛叶皮层、鼻周皮层、梨状皮层、无颗粒岛叶皮层尾部、初级体感皮层桶状区、次级体感皮层、初级运动皮层等。结论：本研究结果说明中枢神经系统内有广泛区域参与了对腮腺分泌的调控。

关键词: 腮腺；  , 神经环路；  , 伪狂犬病毒；  , 下泌涎核；  , 唾液反射

Abstract: Object: To explore the spatial distribution of CNS (central nervous system) nuclei those innervate parotid gland in mice. Methods: Trace amount of pseudorabies virus (PRV) was injected into the parotid gland of three C57BL / 6 mice. The distribution of fluorescently labeled neurons in the brain was observed under microscope after surviving for 5 days. Results: Five days after infection, the lnuclei were abeled as follow. In rhomb encephalon, the labeled-neurons were found in inferior salivatory nucleus (IS), reticular structure, solitary nucleus (Sol), spinal trigeminal nucleus (Sp5), facial nucleus(7N), raphe nuclei(Rn), locus coeruleus (LC), subcoeruleus nucleus(Sub), supratrigeminal nucleus(Su5), parabrachial nucleus(PBN). In mesencephalon and diencephalon, marked cells were found in periaqueductal gray periaqueductal gray(PAG), reticular formation, parasubthalamic nucleus(PSTh), zona incerta (ZI), lateral hypothalamic area(LH),paraventricular hypolhalamic nucleus, posterior part(PaPo), medial preoptic nucleus(MP). In telencephalon, positive neurons were found in central amygdaloid nucleus(CeA), bed nucleus of the stria terminalis (BNST), lateral septal nucleus(LS), dorsolateral entorhinal cortex(DLEnt), granular insular cortex(GI), dysgranular insular cortex(DI), perirhinal cortex(PRh), piriform cortex(Pir), agranular insular cortex, posterior part(AIP), primary somatosensory cortex barrel field(SIBF), secondary somatosensory cortex(S2), primary motor cortex(M1). Conclusion: The results suggest that widespread areas of the CNS are involved in control of parotid gland salivation.

Key words: parotid gland, neural circuits, pseudorabies virus, inferior salivatory nucleus, salivary reflex

中图分类号: 

• R782.4