Abstract:

Objective: To explore the effect and mechanism of necroptosis on the immune microenvironment of periodontitis. Methods: We screened out the differentially expressed necroptosis-related genes in periodontitis, first calculated the hub genes through machine learning algorithms, and constructed a diagnostic model. Then the relative abundance of immune cells in the periodontitis and healthy groups was analyzed and its correlation with necroptosis-related genes was calculated. Collect healthy and inflammatory gingival tissues from individuals and extract RNA, then validate the inter-group expression level of necroptosis-related genes using real-time quantitative polymerase chain reaction (RT-qPCR). Results: A total of 10 necroptosis-related genes were differentially expressed, including 8 hub genes. Compared with healthy tissues, the relative infiltration of plasma cells, B cells, neutrophils and NK cells in periodontal inflammatory tissues were increased. The relative infiltration of macrophages and resting dendritic cells decreased. Among them, absent in melanoma 2 (AIM2), mixed lineage kinase domain like pseudokinase (MLKL) were significantly positively correlated with the expression of plasma cells. Conclusion: Necroptosis affects the immune microenvironment of periodontitis.

Key words: necroptosis, periodontitis, gene microarray, immune microenvironment

摘要：

目的：探索坏死性凋亡对牙周炎免疫微环境的影响及机制初探。方法：筛选出牙周炎中差异表达的坏死性凋亡相关基因，通过机器学习算法计算其中的核心基因并构建诊断模型。分别分析牙周炎与健康组中的免疫细胞相对丰度，并计算其与坏死性凋亡相关基因的相关性。收集人健康牙龈与炎症牙龈组织，并提取其RNA，通过实时定量聚合酶链反应（real-time quantitative polymerase chain reaction，RT-qPCR) 验证坏死性凋亡核心基因的组间表达。结果：共有10个坏死性凋亡相关基因差异表达，其中核心基因共有8个，炎症组织相对于健康组织，浆细胞、B细胞、中性粒细胞与NK细胞含量升高，而巨噬细胞、休眠树突状细胞含量下降。其中，混合系激酶域样伪激酶（mixed lineage kinase domain like pseudokinase，MLKL)、黑色素瘤缺乏因子2 (absent in melanoma 2，AIM2)与浆细胞表达量呈显著正相关。结论：坏死性凋亡对牙周炎免疫微环境存在影响。

关键词: 坏死性凋亡, 牙周炎, 基因芯片, 免疫微环境

CLC Number: 

• R782