《Journal of Oral and Maxillofacial Surgery》 ›› 2023, Vol. 33 ›› Issue (5): 292-297. doi: 10.12439/kqhm.1005-4979.2023.05.003

• Basic Scientific Study • Previous Articles     Next Articles

Preparation of hyaluronic acid injectable hydrogel with SHED-derived exosomes and its anti-inflammatory and osteogenic effects on periodontitis: An experimental study in the rat

YING Qiao(), YU Yiqiang, SU Jiansheng()   

  1. Department of Prosthodontics, Stomatological Hospital and Dental School of Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China
  • Received:2022-01-21 Accepted:2022-05-04 Online:2023-10-28 Published:2023-11-03

负载脱落乳牙干细胞外泌体的透明质酸可注射水凝胶的制备及其对小鼠牙周炎抗炎成骨的研究

应乔(), 俞懿强, 苏俭生()   

  1. 同济大学口腔医学院,同济大学附属口腔医院口腔修复科,上海牙组织修复与再生工程技术研究中心,上海 200072
  • 通讯作者: 苏俭生,教授. E-mail:sjs@tongji.edu.cn
  • 作者简介:
    应乔,住院医师. E-mail:
  • 基金资助:
    国家自然科学基金(81873715); 上海市科学技术委员会项目(18441902100)

Abstract:

Objective: Preparation of injectable hyaluronic acid (HA) hydrogels loaded with exosomes (EXO) of stem cells from human exfoliated deciduous teeth (SHED) to investigate its anti-inflammatory and osteogenic effects in periodontitis. Methods: SHED EXO was extracted and loaded with high molecular weight HA as matrix to synthesize injectable HA hydrogel (SHED-HA) and its degradation rate was determined. The biocompatibility of SHED-HA was analyzed by CCK-8 method, and the osteogenic induction and inflammatory inhibition of SHED-HA were studied by real-time quantitative polymerase chain reaction(RT-qPCR). Subsequently, the effect of SHED-HA on alveolar bone defect in mice was analyzed by micro-CT.  Results: SHED-HA had a suitable degradability at the concentration of 6% w/v HA. CCK-8 results showed that SHED-HA had good biocompatibility. RT-qPCR results showed that the expression levels of inflammatory genes IL-1β and IL-6 were down-regulated in the SHED-HA group, while the expression levels of osteogenic differentiation related genes Runx2,OPN and OCN were up-regulated. In vivo experiments showed that SHED-HA reduced alveolar bone destruction in mice. Conclusion: The HA hydrogel SHED-HA of SHED exosome was successfully constructed, and it was found that SHED-HA has anti-inflammatory and osteogenic effects on mouse periodontitis.

Key words: hyaluronic acid, deciduous tooth stem cells, exosome, osteogenic differentiation

摘要:

目的:制备负载人脱落乳牙干细胞(stem cells from human exfoliated deciduous teeth,SHED)外泌体(exosomes,EXO)的可注射透明质酸(hyaluronic acid,HA)水凝胶,探究其在牙周炎中的抗炎及成骨作用。方法:提取SHED外泌体,以高分子量HA为基质,在其中负载SHED外泌体以合成可注射HA水凝胶(SHED-HA),并测定其降解率。采用CCK-8法分析SHED-HA的生物相容性,应用实时定量聚合酶链反应(real-time quantitative polymerase chain reaction,RT-qPCR)研究SHED-HA的成骨诱导作用及炎症抑制作用。随后,通过micro-CT来分析SHED-HA对小鼠牙槽骨缺损的挽救作用。结果:6% w/v透明质酸水凝胶具有较合适的降解性。CCK-8结果显示,SHED-HA具有较好的生物相容性能。RT-qPCR结果显示,SHED-HA组炎症基因IL-1β、IL-6的表达水平下调,而成骨分化相关基因Runx2、OPN、OCN的表达水平上调。体内实验显示,SHED-HA能减轻小鼠牙槽骨破坏。结论:成功构建负载了SHED外泌体的HA水凝胶SHED-HA,同时证实了其对小鼠牙周炎具有抗炎、成骨的作用。

关键词: 透明质酸, 脱落乳牙干细胞, 外泌体, 成骨分化

CLC Number: