Abstract: Objective: The present study was aimed to study the effects of transforming growth factor -β1 (TGF-β1) in osteoblast-like cell line MC3T3-E1 growth and differentiation after recombinant human bone morphogenetic protein-2 (rhBMP-2) stimulating. Methods: MC3T3-E1 was grown in DMEM supplemented with 10%FBS. Logarithmic growth of MC3T3-E1 were divided into 3 groups as follows: (Group 1) DMEM medium (control), (Group 2) rhBMP-2 in different concentrations, and (Group 3) TGF-β1+ rhBMP-2 in different concentrations. Cell viability was measured by MTT assay. ALP activity was evaluated by Elisa test. We used analysis of variance (SPSS 16.0 for Windows software) to determine the differences observed among the groups; α=0.05 was considered significant. Results: The number of MC3T3-E1 cells increased with rhBMP-2 in a concentration-dependent manner. A significantly increased number of MC3T3-E1 cells was found at TGF-β1 + rhBMP-2 group (P<0.05) in which also exhibited in a rhBMP-2 concentration-dependent manner. rhBMP-2 treated MC3T3-E1 cells had intensively enhanced ALP activity and expressed more significant in TGF-β1+ rhBMP-2 group (P<0.01). Conclusion: The findings suggest that TGF-β1+rhBMP-2 or rhBMP-2 alone induce osteoblastic differentiation of MC3T3-E1 cell line.

Key words: TGF-&beta, 1； rhBMP-2； MC3T3-E1；osteogenesis

摘要： 目的： 观察TGF-β1对rhBMP-2促进成骨前体细胞（MC3T3-E1）增殖和分化能力的影响。方法： MC3T3-E1加入不同浓度的rhBMP-2和TGF-β1+ rhBMP-2进行组织学观察，用MTT法检测细胞增殖，通过ELISA检测细胞ALP含量。结果： 随着加入rhBMP-2浓度的增加，MC3T3-E1细胞的增殖逐渐增加，TGF-β1+rhBMP-2组增加更为显著（P<0.05）。TGF-β1+rhBMP-2组和rhBMP-2组在rhBMP-2浓度为25、50、100 μg/L间存在统计学意义（P<0.05）。随着rhBMP-2浓度的增加，ALP值逐渐增加，TGF-β1+rhBMP-2组ALP值升高更为显著（P<0.01），TGF-β1+rhBMP-2组和rhBMP-2组在rhBMP-2浓度为25、50 μg/L间有统计学意义（P <0.05）。结论： TGF-β1可促进rhBMP-2对MC3T3-E1的增殖和成骨分化作用。

关键词: TGF-&beta, 1； rhBMP-2； MC3T3-E1；成骨

CLC Number: 

• Q813.11

• R329.28