《Journal of Oral and Maxillofacial Surgery》 ›› 2014, Vol. 24 ›› Issue (4): 276-. doi: 10.3969/j.issn.1005-4979.2014.04.006

• Basic Scientific Study • Previous Articles     Next Articles

Effect of Icariin on the Proliferation,Osteogenic Differentiation, and Adipogenic Differentiation of Canine Bone Marrow Mesenchymal Stem Cells

LIU Tian-lin1,2, ZHANG Xue-bin1, ZHANG Xin1, PAN Ting2, WU Yan-ling2, HUANG Yuan-liang2   

  1. 1. Laboratory of Oral Biomedical Science and Translational Medicine, School of Stomatology, Tongji University, Shanghai 200072; 2. Department of Stomatology, Oriental Hospital, Tongji University, Shanghai 200120; 3. Department of Dental Implant, Yantai Hospital of Stomatology, Yantai 264008, Shandong Province, China
  • Online:2014-08-28 Published:2021-11-04

淫羊藿苷对犬骨髓间充质干细胞增殖、成骨和成脂分化的影响

刘天麟1,2,张学斌1,3,张忻1,潘汀2,吴艳玲2,黄远亮2   

  1. 1. 同济大学口腔医学院口腔生物医学及转化医学实验室,上海   200072;2. 同济大学附属上海市东方医院口腔科,上海   200120;3. 烟台市口腔医院口腔种植科,山东   烟台   264008
  • 通讯作者: 黄远亮,教授,主任医师. E-mail:13301921076@163.com
  • 作者简介:刘天麟(1980—),男,安徽人,博士研究生,主治医师. E-mail: 525ya@163.com
  • 基金资助:

    国家自然科学基金资助项目(81070806);上海市东方医院东方朝阳人才计划项目(DFZY-09)

Abstract: Objective: To investigate the effects of icariin on the proliferation and osteogenic / adipogenic differentiation of canine bone marrow mesenchymal stem cells (BMSCs). Methods: Canine BMSCs were treated and cultured with icariin in concentrations of 1×10-9, 1×10-8, 1×10-7, 1×10-6, 1×10-5, 1×10-4 mol/L for experiment, and 0 mol/L was used as a blank control. To compare the in vitro osteogenic / adipogenic differentiation ability of BMSCs incubated in osteogenic / adipogenic medium, we performed a CCK-8 assay for cell proliferation, an alkaline phosphatase (ALP) activity assay, a calcium deposition assay (alizarin red staining, ARS), and a lipids deposition assay (oil red O staining). Results: Icariin promoted proliferation of canine BMSCs in 1×10-6 mol/L concentration, and 1×10-4 mol/L concentration icariin inhibited proliferation.  The relation between canine BMSCs ALP activity and icariin concentration demonstrated in a dose-dependent manner. After culture in nonosteogenic / adipogenic medium, there were no significant difference between calcium deposition and lipids deposition in all groups. But after culture in osteogenic / adipogenic medium, proportion of calcium deposition in Icariin group(1×10-6mol/L) was higher than control group. On the contrary, proportion of lipid in icariin group(1×10-6mol/L) was lower than the control group. Conclusion: Icariin can promote the proliferation and osteogenic differentiation of canine BMSCs and inhibit adipogenic differentiation.

Key words: icariin; , BMSCs; , osteogenic differentiation; , adipogenic differentiation

摘要: 目的:研究淫羊藿苷对犬骨髓间充质干细胞(bone marrow mesenchymal stem cells ,BMSCs)增殖和成骨、成脂分化的影响。方法:将淫羊藿苷配制成终浓度为1×10-9、1×10-8、1×10-7、1×10-6、1×10-5、1×10-4 mol/L的溶液,以0 mol/L组为空白对照组,分别用于体外培养犬BMSCs。CCK-8试剂盒检测细胞增殖情况,碱性磷酸酶试剂盒检测碱性磷酸酶活性。然后实验分为淫羊藿苷组和对照组,分别在含或不含成骨、成脂诱导液条件下,行成骨、成脂诱导分化实验,通过茜素红和油红O染色检测各组钙沉积和脂质形成面积。结果:发现淫羊藿苷1×10-6 mol/L组促进犬BMSCs增殖,而1×10-4 mol/L组抑制增殖。碱性磷酸酶活性与淫羊藿苷浓度呈剂量依赖性关系。不含成骨、成脂诱导液条件下,各组均几乎未见明显钙沉积和脂质形成;含成骨、成脂诱导液条件下,淫羊藿苷组(1×10-6 mol/L)钙沉积面积高于对照组,脂质形成面积低于对照组。结论:淫羊藿苷能促进犬BMSCs增殖和成骨分化,并抑制其成脂分化。

关键词: 淫羊藿苷;  , 骨髓间充质干细胞;  , 成骨分化;  , 成脂分化;  ,

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