Abstract: Objective: To investigate the effects of icariin on the proliferation and osteogenic / adipogenic differentiation of canine bone marrow mesenchymal stem cells (BMSCs). Methods: Canine BMSCs were treated and cultured with icariin in concentrations of 1×10-9, 1×10-8, 1×10-7, 1×10-6, 1×10-5, 1×10-4 mol/L for experiment, and 0 mol/L was used as a blank control. To compare the in vitro osteogenic / adipogenic differentiation ability of BMSCs incubated in osteogenic / adipogenic medium, we performed a CCK-8 assay for cell proliferation, an alkaline phosphatase (ALP) activity assay, a calcium deposition assay (alizarin red staining, ARS), and a lipids deposition assay (oil red O staining). Results: Icariin promoted proliferation of canine BMSCs in 1×10-6 mol/L concentration, and 1×10-4 mol/L concentration icariin inhibited proliferation.  The relation between canine BMSCs ALP activity and icariin concentration demonstrated in a dose-dependent manner. After culture in nonosteogenic / adipogenic medium, there were no significant difference between calcium deposition and lipids deposition in all groups. But after culture in osteogenic / adipogenic medium, proportion of calcium deposition in Icariin group（1×10-6mol/L） was higher than control group. On the contrary, proportion of lipid in icariin group（1×10-6mol/L） was lower than the control group. Conclusion: Icariin can promote the proliferation and osteogenic differentiation of canine BMSCs and inhibit adipogenic differentiation.

Key words: icariin; , BMSCs; , osteogenic differentiation; , adipogenic differentiation

摘要： 目的：研究淫羊藿苷对犬骨髓间充质干细胞（bone marrow mesenchymal stem cells ，BMSCs)增殖和成骨、成脂分化的影响。方法：将淫羊藿苷配制成终浓度为1×10-9、1×10-8、1×10-7、1×10-6、1×10-5、1×10-4 mol/L的溶液，以0 mol/L组为空白对照组，分别用于体外培养犬BMSCs。CCK-8试剂盒检测细胞增殖情况，碱性磷酸酶试剂盒检测碱性磷酸酶活性。然后实验分为淫羊藿苷组和对照组，分别在含或不含成骨、成脂诱导液条件下，行成骨、成脂诱导分化实验，通过茜素红和油红O染色检测各组钙沉积和脂质形成面积。结果：发现淫羊藿苷1×10-6 mol/L组促进犬BMSCs增殖，而1×10-4 mol/L组抑制增殖。碱性磷酸酶活性与淫羊藿苷浓度呈剂量依赖性关系。不含成骨、成脂诱导液条件下，各组均几乎未见明显钙沉积和脂质形成；含成骨、成脂诱导液条件下，淫羊藿苷组（1×10-6 mol/L）钙沉积面积高于对照组，脂质形成面积低于对照组。结论：淫羊藿苷能促进犬BMSCs增殖和成骨分化，并抑制其成脂分化。

关键词: 淫羊藿苷；  , 骨髓间充质干细胞；  , 成骨分化；  , 成脂分化；  , 犬

CLC Number: 

• R782.2

• Q813.1