《Journal of Oral and Maxillofacial Surgery》

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Cytocompatibility of Pure Magnesium Anodizing-silane/phytic Acid Double Composite Zinc Coating

MING Kun1, LI De-chao2, LI Mu-qin1, WANG Xin-yu2, ZHANG Hui-ming3, JIAO Yu-feng1   

  1. (1. Stomatological College of Jiamusi University, Jiamusi 154007; 2. Key Laboratory of Biomedical Materials, Jiamusi University, Jiamusi 154007; 3. Experimental Center of Life Sciences, College of Basic Medical Sciences, Jiamusi University, Jiamusi 154007, Heilongjiang Province, China)
  • Online:2018-02-28 Published:2019-04-16

纯镁复合硅烷/植酸载锌膜层的细胞相容性实验研究

明堃1, 李德超2, 李慕勤1, 王心彧2, 张慧明3, 焦玉凤1   

  1. (1. 佳木斯大学口腔医学院,黑龙江 佳木斯 154007;2. 佳木斯大学黑龙江省高校生物医学材料重点实验室,黑龙江 佳木斯 154007;3. 佳木斯大学基础医学院生命科学实验中心,黑龙江 佳木斯 154007)
  • 通讯作者: 李慕勤(1955—),女,黑龙江绥化人,教授,博士. E-mail: jmsdxlimuqin@163.com
  • 作者简介:明堃(1991—),女,河北张家口人,硕士研究生. E-mail: 158549215@qq.com
  • 基金资助:
    国家自然科学基金(31370979);佳木斯大学协同创新中心(2011xtcx2016-02)

Abstract: Objective: To prepare pure magnesium anodic oxidation-silane/phytic acid double composite zinc coating, hoping to improve the corrosion resistance and cellular biocompatibility of pure magnesium. Method: To prepare pure magnesium anodic oxidation, anodic oxidation-silane treatment, silane/phytic acid double compound treatment and silane/phytic acid double compound loaded Zn specimens, observing the morphology of the coating surface and analyze the main elements by SEM, detecting the cell proliferation and adhesion ability by CCK-8, detecting the cell adhesion morphology by Confocal laser. Results: There were micropores on the surface of pure magnesium anodizing and the composite treatment played a role in sealing. Zinc loaded with different concentrations increased the cell proliferation and adhesion ability. Anodic oxidation-silane/phytic acid double composite loading Zn 20 g/L could prevent cell fluid immersion and the biocompatibility was the best. Conclusion: Pure magnesium anodizing-silane/phytic acid double composite loading Zn 20 g/L film, cell growth and morphology spread best, significantly promoted the osteoblast proliferation, adhesion ability and biological function expression on the surface of the material.

Key words: pure magnesium, anode oxidation, silane/ phytic acid, zinc content, cell compatibility

摘要: 目的:制备纯镁复合硅烷/植酸载锌膜层,希望可以改善纯镁的耐腐蚀性及其细胞生物相容性。方法:制备纯镁阳极氧化、阳极氧化-硅烷处理、阳极氧化-硅烷/植酸双复合处理及硅烷/植酸双复合载Zn试样,采用扫描电镜观察涂层表面形貌,并进行分析元素,通过CCK-8检测细胞增殖和黏附能力,激光共聚焦检测细胞黏附形态。结果:纯镁阳极氧化表面存在微孔,复合处理起到封孔的作用,载不同浓度锌增加了细胞增殖和黏附能力,其中阳极氧化-硅烷/植酸双复合载Zn 20 g/L可防止细胞液浸入,生物相容性最佳。结论:纯镁阳极氧化-硅烷/植酸双复合载Zn 20 g/L膜层,细胞增长和形态铺展最好,明显促进了成骨细胞在材料表面的增殖、黏附能力及其生物功能表达。

关键词: 纯镁, 阳极氧化, 硅烷/植酸, 锌含量, 细胞相容性

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