《Journal of Oral and Maxillofacial Surgery》 ›› 2014, Vol. 24 ›› Issue (1): 10-. doi: 10.3969/j.issn.1005-4979.2014.01.003

• Basic Scientific Study • Previous Articles     Next Articles

Oral Care and Collagen Metabolism in the Development of CIGO

ZHAO Jing, GAO Ming-jing, JIANG Lei, ZHAO Yun-fu   

  1. Department of Stomatology, Changzheng Hospital, Second Military Medical
    University, Shanghai 200003, China
  • Online:2014-02-28 Published:2014-06-27

环孢素A致牙龈增生与菌斑、胶原代谢的相关性研究

赵婧,   高明津,   姜蕾,   赵云富   

  1. 第二军医大学附属长征医院口腔科,上海   200003
  • 通讯作者: 赵云富,教授. E-mail:zhaoyf1818@126.com
  • 作者简介:赵婧(1985—),女,黑龙江人,讲师,硕士.E-mail: joanjing1985@126.com
  • 基金资助:

    2011年国家自然基金资助项目(81170966)

Abstract: Objective: The aim of the study was to investigate the role of the plaque formation on the development of cyclosporine A induced gingival overgrowth (CIGO). Methods:  40 Sprague-Dawley rats were selected and subjected to all left maxillary molars extraction. All rats were randomly divided into two groups. Rats in the test group (n=20) received cyclosporine A (CsA), which was dissolved in olive oil via gastric feeding (30 mg/kg body weight every day) to establish a rat model of the CIGO. Rats in the control group (n=20) received olive oil only. After 4 weeks, rats were sacrificed and gingival tissues were observed by macroscopic and microscopic methods. The expressions of CyPA, CD147 and MMP-2 in gingival tissues were evaluated by immunohistochemistry and Real-time PCR. Results:  In the CsA-treated animals, both sides of the maxillary gingival were found to be enlarged and the body weights reduced. In the control group, expressions of CyPA and MMP-2 at protein and mRNA levels were obviously higher than that in test group(P<0.01). But no significant difference was found in CD147 expression between two groups(P>0.05). Conclusion:  Oral care manifested as plaque formation is the relevant factor but not initiating factor of CIGO. The mechanism of CIGO may be concerned with the formation of CsA-CyPA complex and competitive inhibition of the interaction between CyPA and CD147, leading to  imbalance of collagen degradation and accumulation, which causes gingival overgrowth.

Key words: cyclosporin A, gingival overgrowth; , plaque, matrix metalloproteinase-2; , CD147; , cyclophilin A

摘要:  目的:探讨菌斑存在与否对环孢素A(CsA)所致牙龈增生(CIGO)的影响及其可能的发病机制。方法:SD雄性大鼠40只,实验组、对照组各20只,均拔除左侧上颌磨牙,在同一SD大鼠口内建立有/无菌斑条件下的牙龈模型。实验组每天胃饲含CAS的橄榄油溶液(30 mg/kg),对照组胃饲等量纯橄榄油。定期观察两组大鼠牙龈增生情况,运用免疫组化和RT?鄄PCR方法观察两组大鼠牙龈内CyPA、CD147、MMP?鄄2的表达差异。结果:实验组上颌双侧牙龈增生明显,同一大鼠口内拔牙侧和非拔牙侧牙龈增生程度无显著差异; CyPA和MMP?鄄2的蛋白和mRNA表达水平实验组较对照组均明显减少(P<0.01);CD147的蛋白质和mRMA表达水平在两组间均无显著差异(P>0.05);实验组和对照组中有牙侧和无牙侧CyPA、CD147和MMP?鄄2的蛋白质和mRMA表达水平均无显著差异(P>0.05)。结论:即使无菌斑存在,仍有CIGO发生; CyPA与MMP?鄄2表达的下降,可能是牙龈组织中胶原降解减少、牙龈增生的原因之一。

关键词:  环孢素A;  , 牙龈增生;  , 菌斑;  , 基质金属蛋白酶-2;  , CD147;  , 亲环素A

CLC Number: