Spatial Distribution of the Central Nervous System Nuclei that Innervate the Parotid Gland in Mice

doi:10.3969/j.issn.1005-4979.2019.03.001

Abstract

Abstract: Object: To explore the spatial distribution of CNS (central nervous system) nuclei those innervate parotid gland in mice. Methods: Trace amount of pseudorabies virus (PRV) was injected into the parotid gland of three C57BL / 6 mice. The distribution of fluorescently labeled neurons in the brain was observed under microscope after surviving for 5 days. Results: Five days after infection, the lnuclei were abeled as follow. In rhomb encephalon, the labeled-neurons were found in inferior salivatory nucleus (IS), reticular structure, solitary nucleus (Sol), spinal trigeminal nucleus (Sp5), facial nucleus(7N), raphe nuclei(Rn), locus coeruleus (LC), subcoeruleus nucleus(Sub), supratrigeminal nucleus(Su5), parabrachial nucleus(PBN). In mesencephalon and diencephalon, marked cells were found in periaqueductal gray periaqueductal gray(PAG), reticular formation, parasubthalamic nucleus(PSTh), zona incerta (ZI), lateral hypothalamic area(LH),paraventricular hypolhalamic nucleus, posterior part(PaPo), medial preoptic nucleus(MP). In telencephalon, positive neurons were found in central amygdaloid nucleus(CeA), bed nucleus of the stria terminalis (BNST), lateral septal nucleus(LS), dorsolateral entorhinal cortex(DLEnt), granular insular cortex(GI), dysgranular insular cortex(DI), perirhinal cortex(PRh), piriform cortex(Pir), agranular insular cortex, posterior part(AIP), primary somatosensory cortex barrel field(SIBF), secondary somatosensory cortex(S2), primary motor cortex(M1). Conclusion: The results suggest that widespread areas of the CNS are involved in control of parotid gland salivation.

Key words: parotid gland, neural circuits, pseudorabies virus, inferior salivatory nucleus, salivary reflex

摘要： 目的：探究调控腮腺分泌的中枢神经系统内相关核团的分布。方法：选取8~12周龄C57BL/6小鼠共3只，在小鼠腮腺分3个位点注射伪狂犬病毒重组体（PRV） 3μL，注射病毒5 d后荧光显微镜下观察小鼠大脑中被标记的核团分布。结果：PRV感染5 d后，被标记的脑区在后脑主要包括下泌涎核、后脑区的网状结构、孤束核、三叉神经脊束核、面神经核、中缝核群、蓝斑、蓝斑下核、三叉神经上核、臂旁核等；中脑及间脑主要有中脑导水管周围灰质、中脑区的网状结构、底丘脑旁核、未定带、下丘脑外侧区、下丘脑室旁核尾部、视前内侧区等；端脑中有中央杏仁核、终纹床核、外侧隔核、背外侧内嗅皮层、颗粒状岛叶皮层、异粒状岛叶皮层、鼻周皮层、梨状皮层、无颗粒岛叶皮层尾部、初级体感皮层桶状区、次级体感皮层、初级运动皮层等。结论：本研究结果说明中枢神经系统内有广泛区域参与了对腮腺分泌的调控。

关键词: 腮腺； , 神经环路； , 伪狂犬病毒； , 下泌涎核； , 唾液反射

CLC Number:

R782.4

ZHANG Yu han, HAN Wen fei, MENG Bo, SU Jian sheng, XIAO Fan. Spatial Distribution of the Central Nervous System Nuclei that Innervate the Parotid Gland in Mice[J]. 《Journal of Oral and Maxillofacial Surgery》, doi: 10.3969/j.issn.1005-4979.2019.03.001.

张雨菡，韩雯斐，孟博，苏俭生，肖凡. 调控小鼠腮腺分泌中枢神经系统相关核团的分布[J]. 《口腔颌面外科杂志》, doi: 10.3969/j.issn.1005-4979.2019.03.001.

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URL: https://journal06.magtech.org.cn/Jweb_joms/EN/10.3969/j.issn.1005-4979.2019.03.001

https://journal06.magtech.org.cn/Jweb_joms/EN/Y2019/V29/I3/121

References

[1] Dawes C, Pedersen AM, Villa A, et al. The functions of human saliva: A review sponsored by the world workshop on oral medicine VI[J]. Arch Oral Biol, 2015, 60(6):863-874.
[2] Matsuo R. Role of saliva in the maintenance of taste sensitivity[J]. Crit Rev Oral Biol Med, 2000, 11(2):216-229.
[3] Suwabe T, Fukami H, Bradley RM. Synaptic responses of neurons controlling the parotid and von Ebner salivary glands in rats to stimulation of the solitary nucleus and tract[J]. J Neurophysiol, 2008, 99(3):1267-1273.
[4] 任小红. 唾液的分泌及影响因素[J]. 中国实用医药, 2009, 4(20):252-254.
[5] 吴圆琴, 付纪, 宋韬, 等. 口干症的病因及治疗进展[J]. 临床口腔医学杂志, 2015, 31(12):762-764.
[6] Ekstr?觟m J, Khosravani N, Castagnola M, et al. Saliva and the control of its secretion[M]//Ekberg O. Dysphagia, Medical Radololgy. Berlin Heidelberg: Springer, 2011:19-47.
[7] Garrett JR. The proper role of nerves in salivary secretion: a review[J]. J Dent Res, 1987, 66(2):387-397.
[8] Ekstrand MI, Enquist LW, Pomeranz LE. The alpha-herpesviruses: molecular pathfinders in nervous system circuits[J]. Trends Mol Med, 2008, 14(3):134-140.
[9] Card JP, Enquist LW. Transneuronal circuit analysis with pseudorabies viruses[J]. Curr Protoc Neurosci, 2014, 68:1.5.1-1.539.
[10] Rezek O, Boldogkoi Z, Tomb?觃cz D, et al. Location of parotid preganglionic neurons in the inferior salivatory nucleus and their relation to the superior salivatory nucleus of rat[J]. Neurosci Lett, 2008, 440(3):265-269.
[11] Satoh-Kuriwada S, Shoji N, Miyake H, et al. Effects and mechanisms of tastants on the gustatory-salivary reflex inhuman minor salivary glands[J]. Biomed Res Int, 2018, 2018:3847075.
[12] Maeda N, Kobashi M, Mitoh Y, et al. Differential involvement of two cortical masticatory areas in submandibular salivary secretion in rats[J]. Brain Res, 2014, 1543:200-208.
[13] Morquette P, Lavoie R, Fhima MD, et al. Generation of the masticatory central pattern and its modulation by sensory feedback[J]. Prog Neurobiol, 2012, 96(3):340-355.
[14] Matsuo R, Yamauchi Y, Kobashi M, et al. Role of parabrachial nucleus in submandibular salivary secretion induced by bitter taste stimulation in rats[J]. Auton Neurosci, 2001, 88(1-2):61-73.
[15] Matsuo R, Yamamoto T, Yoshitaka K, et al. Neural substrates for reflex salivation induced by taste, mechanical, and thermal stimulation of the oral region in decerebrate rats[J]. Jpn J Physiol, 1989, 39(3):349-357.
[16] Lundy RF Jr, Norgren R. Activity in the hypothalamus, amygdala, and cortex generates bilateral and convergent modulation of pontine gustatory neurons[J]. J Neurophysiol, 2004, 91(3):1143-1157.
[17] Matsuo R, Kusano K. Lateral hypothalamic modulation of the gustatory-salivary reflex in rats[J]. J Neurosci, 1984, 4(5):1208-1216.
[18] Grossman SP, Dacey D, Halaris AE, et al. Aphagia and adipsia after preferential destruction of nerve cell bodies in hypothalamus[J]. Science, 1978, 202(4367):537-539.
[19] Galaverna OG, Seeley RJ, Berridge KC, et al. Lesions of the central nucleus of the amygdala. I: Effects on taste reactivity, taste aversion learning and sodium appetite[J]. Behav Brain Res, 1993, 59(1-2):11-17.
[20] Zhang GX, Sasamoto K. Projections of two separate cortical areas for rhythmical jaw movements in the rat[J]. Brain Res Bull, 1990, 24(2):221-230.
[21] Barbier M, Chometton S, Peterschmitt Y, et al. Parasubthalamic and calbindin nuclei in the posterior lateral hypothalamus are the major hypothalamic targets for projections from the central and anterior basomedial nuclei of the amygdala[J]. Brain Struct Funct, 2017, 222(7):2961-2991.
[22] McCue DL, Kasper JM, Hommel JD. Regulation of motivation for food by neuromedin U in the paraventricular nucleus and the dorsal raphe nucleus[J]. Int J Obes (Lond), 2017, 41(1):120-128.
[23] Hiraba H, Yamaoka M, Fukano M, et al. Increased secretion of salivary glands produced by facial vibrotactile stimulation[J]. Somatosens Mot Res, 2008, 25(4):222-229.
[24] Gholami N, Hosseini Sabzvari B, Razzaghi A, et al. Effect of stress, anxiety and depression on unstimulated salivary flow rate and xerostomia[J]. J Dent Res Dent Clin Dent Prospects, 2017, 11(4):247-252.
[25] Godoy LD, Rossignoli MT, Delfino-Pereira P, et al. A comprehensive overview on stress neurobiology: basic concepts and clinical implications[J]. Front Behav Neurosci, 2018, 12:127.
[26] Staiger JF, Nürnberger F. The efferent connections of the lateral septal nucleus in the guinea pig: intrinsic connectivity of the septum and projections to other telencephalic areas[J]. Cell Tissue Res, 1991, 264(3):415-426.
[27] Kim HI, Kim YY, Chang JY, et al. Salivary cortisol, 17β-estradiol, progesterone, dehydroepiandrosterone, and α-amylase in patients with burning mouth syndrome[J]. Oral Dis, 2012, 18(6):613-620.
[28] Herman JP, McKlveen JM, Ghosal S, et al. Regulation of the hypothalamic-pituitary-adrenocortical stress response[J]. Compr Physiol, 2016, 6(2):603-621.
[29] Suzuki WA, Naya. The perirhinal cortex[J]. Annu Rev Neurosci, 2014, 37:39-53.
[30] Moser EI, Moser MB. Grid cells and neural coding in high-end cortices[J]. Neuron, 2013, 80(3):765-774.
[31] Courtiol E, Wilson DA. The olfactory mosaic: bringing an olfactory network together for odor perception[J]. Perception, 2017, 46(3-4):320-332.

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