VEGF-ASODN转染SACC-2细胞对其VEGF表达及生长的影响

doi:10.3969/j.issn.1005-4979.2013.01.004

《口腔颌面外科杂志》 ›› 2013, Vol. 23 ›› Issue (1): 18-22. doi: 10.3969/j.issn.1005-4979.2013.01.004

VEGF-ASODN转染SACC-2细胞对其VEGF表达及生长的影响

李晓光¹，　李腾宇²，　王旭霞³，　王延秀⁴，　肖长杰¹，　徐晓⁴，　李俊福³

1.泰安市中心医院口腔科，山东泰安271000； 2.青岛大学医学院口腔系，山东青岛266071； 3.山东大学口腔医学院，山东济南250012；4.泰安市中心医院麻醉科, 山东泰安271000

出版日期:2013-02-28 发布日期:2013-05-08
通讯作者: 李晓光，主任医师． E-mail: lxg0538@163.com
作者简介:李晓光（1962—），男，浙江海盐人，主任医师，博士.
基金资助:
山东省科学技术发展计划资助项目（2011GGH21822）

Expression and Significance of VEGF in Salivary Adenoid Cystic Carcinoma Cells in Association with VEGF-ASODN Transfection

LI Xiao-guang¹， LI Teng-yu², WANG Xu-xia³, WANG Yan-xiu4, XIAO Chang-jie¹, XU Xiao⁴，LI Jun-fu³

1. Department of Stomatology，Tai′an Municipal Central Hospital，Tai′an 271000；
2. Department of Stomatology，Qingdao University Medical College, Qingdao 266071；
3. Shandong University School of Stomatology, Jinan 250012；4. Department of Anesthesiology,
Tai′an Municipal Central Hospital，Tai′an 271000, Shandong Province, China

Online:2013-02-28 Published:2013-05-08

摘要/Abstract

摘要： 目的：研究血管内皮细胞生长因子(VEGF)-反义寡核苷酸(ASODN)转染涎腺腺样囊性癌(salivany adenoid cystic cancinoma, SACC)ACC-2细胞对其VEGF表达和生长的抑制作用。 方法: 人工合成硫代磷酸化VEGF-ASODN，转染SACC-2细胞，24 h后原位杂交及免疫组织化学法检测细胞VEGF mRNA及蛋白表达，ELISA法检测培养液上清中VEGF-ASODN能显著降低VEGF mRNA及蛋白水平、降低培养液中VEGF蛋白含量、增加细胞凋亡、抑制细胞活性及生长。结论: VEGF-ASODN转染SACC-2细胞能显著抑制VEGF mRNA及蛋白表达、增加细胞凋亡、抑制细胞生长。

关键词: 血管内皮细胞生长因子, 反义寡核苷酸, 转染, 涎腺腺样囊性癌ACC-2细胞

Abstract: Objective: The study was designed to observe whether VEGF-antisense oligonucleotide (VEGF-ASODN) transfection to SACC-2 cells are associated with its expression of vascular endothelial growth factor (VEGF) and its′ pathological significances. Methods： The VEGF-ASODN was synthesized artificially with phosphorothioic acid. After transfecting with VEGF-ASODN into SACC-2, the quantity of VEGF mRNA and protein was detected by in situ hybridization and immunohistochemistry. The quantity of VEGF protein in supernatant was detected by ELISA. Flow Cytometry and MTT assay were used to detect cell activity and cellular apoptosis. SPSS 13.0 software was used for statistical analysis. Results: VEGF mRNA protein levels within the cells and in culture medium both decreased significantly after transfected by VEGF-ASODN (P<0.01，P<0.05). The ratio of SACC-2 cell apoptosis was increased. The activities of cells were also decreased after transfected by VEGF-ASODN (P<0.01). Conclusion: Transfection with VEGF-ASODN in SACC-2 can markedly inhibit expression of VEGF. It can enhance cellular apoptosis and suppress pathological growth of these cells.

Key words: vascular endothelial growth factor, antisense oligonucleotide, transfection, salivary adenoid cystic carcinoma cell ACC-2

中图分类号:

R739.8

李晓光，李腾宇，王旭霞，王延秀，肖长杰，徐晓，李俊福. VEGF-ASODN转染SACC-2细胞对其VEGF表达及生长的影响[J]. 《口腔颌面外科杂志》, 2013, 23(1): 18-22.

LI Xiao-guang， LI Teng-yu, WANG Xu-xia, WANG Yan-xiu, XIAO Chang-jie, XU Xiao，LI Jun-fu. Expression and Significance of VEGF in Salivary Adenoid Cystic Carcinoma Cells in Association with VEGF-ASODN Transfection [J]. 《Journal of Oral and Maxillofacial Surgery》, 2013, 23(1): 18-22.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2013/V23/I1/18

参考文献

[1] 邱蔚六.口腔颌面外科学[M]. 6版. 北京:人民卫生出版社，2009：315-316.

[2] Arora S, Kaur J, Sharma C, et al. Stromelysin 3, Ets-1, and vascular endothelial growth factor expression in oral precancerous and cancerous lesions: correlation with microvessel density, progression, and prognosis[J]. Clin Cancer Res，2005,11(6):2272-2284.

[3] Crooke ST. Oligonucleotide analogs might be designed to bind to m-RNA[J]. Anticancer Drug Des，1997,12(5):311-313.

[4] Crooke ST. Vitravene-another piece in the mosaic[J]. Antisense Nucleic Acid Drug Dev，1998,8(4):Vii-Viii.

[5] 李晓光,于肖鹏,刘萍,等.涎腺腺样囊性癌中NOS和VEGF的表达与血管生成及临床病理因素的关系[J]. 临床口腔医学杂志,2011,27（6): 334-337.

[6] 李晓光,于肖鹏,卢会青, 等. VEGF表达在涎腺腺样囊性癌中的意义[J].口腔医学研究，2011,27（6):501-503.

[7] Kamat A, Rajoria S, George A, et al. Estrogen-mediated angiogenesis in thyroid tumor microenvironment is mediated through VEGF signaling pathways[J]. Arch Otol-aryngol Head Neck Surg，2011,137(11):1146-1153.

[8] Nassehi D, Dyrbye H, Andresen M, et al. Vascular endothelial growth factor A protein level and gene expression in intracranial meningiomas with brain edema[J]. APMIS，2011,119(12):831-843.

[9] Chen J, Li T, Wu Y, et al. Prognostic significance of vascular endothelial growth factor expression in gastric carcinoma: a meta-analysis[J]. J Cancer Res Clin Oncol，2011,137(12):1799-1812.

[10] O′Connell JT, Sugimoto H, Cooke VG, et al. VEGF-A and Tenascin-C produced by S100A4+ stromal cells are important for metastatic colonization[J]. Proc Natl Acad Sci USA，2011，108(38):16002-16007.

[11] 李晓光，王延秀，周宝琴，等. VEGF-ASODN转染对舌癌Tca8113细胞VEGF表达及生长的抑制作用[J]. 上海口腔医学，2009,18（5): 509-514.

[12] Usman Saeed M, Batra R, Qureshi F, et al. Reflux of drug during intra-vitreal anti-VEGF therapies[J]. Semin Ophtha-lmol, 2011,26(6):357-360.

[13] Ueta T, Mori H, Kunimatsu A, et al. Stroke and anti-VEGF therapy[J]. Ophthalmology，2011 ,118(10):2093.

[14] 李晓光，王延秀，高德安，等. VEGF反义寡核苷酸对舌鳞癌影响的实验研究[J].口腔颌面外科杂志,2009,19（6): 395-399.

[15] 高渝，肖亚，黄赤兵，等.超声微泡介导VEGF反义寡核苷酸对裸鼠膀胱癌移植瘤生长的抑制作用[J]. 重庆医科大学学报，2009,34（9):1176-1179.

[16] 宋士刚，邢丽娜. VEGF反义核酸联合放射治疗对裸鼠移植瘤宫颈癌的作用[J]. 哈尔滨医科大学学报， 2009, 43（1): 41-44.

[17] 方松清，曾谷清. VEGF反义寡核苷酸与顺铂联合治疗抑制裸鼠骨肉瘤的生长[J]. 南华大学学报：医学版, 2009,37（1):36-38.

[18] 孙杰，吴斌，梁标. 反义VEGF寡核苷酸抑制小鼠肺癌作用的实验研究[J]. 中华肿瘤防治杂志，2008,15（14):1057-1060.

[19] 郑斌，杨策尧，段体德，等. VEGF反义寡核苷酸对裸鼠荷胃癌肿瘤的生长抑制作用研究[J]. 昆明医学院学报，2008,29（1):46-51.

[20] Burke PA, DeNardo SJ. Antiangiogenic agents and their promising potential in combined therapy[J]. Crit Rev Onc-ol Hematol，2001，39(1-2):155-171.

[21] Martinez LA, Naguibneva I, Lehrmann H, et al. Synthetic small inhibiting RNAs: efficient tools to inactivate oncogenic mutations and restore p53 pathways[J]. Proc Natl Acad Sci USA，2002,99(23):14849-14854.

[22] Donzé O, Picard D. RNA interference in mammalian cells using siRNAs synthesized with T7 RNA polymerase[J]. Nucleic Acids Res，2002,30(10):e46.

[23] Dagar S, Krishnadas A, Rubinstein I, et al. VIP grafted sterically stabilized liposomes for targeted imaging of breast cancer: in vivo studies[J]. J Control Release，2003, 91(1-2):123-133.

[24] 邓凌,农晓琳. 口腔颌面头颈鳞癌靶向治疗研究进展[J].口腔颌面外科杂志,2009,19（2): 145-148.

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VEGF-ASODN转染SACC-2细胞对其VEGF表达及生长的影响

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