电离辐射对大鼠颌下腺TNF&alpha;和FGF2表达的影响

doi:10.3969/j.issn.1005-4979.2015.04.003

《口腔颌面外科杂志》 ›› 2015, Vol. 25 ›› Issue (4): 253-. doi: 10.3969/j.issn.1005-4979.2015.04.003

电离辐射对大鼠颌下腺TNFα和FGF2表达的影响

朱红华1，鞠昊1，公柏娟2，李志民1，童辉燕1，马珊珊1，孙宏晨3

1. 吉林大学口腔医院放射科，2. 正畸科，3. 病理科，吉林长春 130021

出版日期:2015-08-28 发布日期:2015-12-01
通讯作者: 李志民，副教授. E-mail:lzmxx@aliyun.com
作者简介:朱红华(1990—)，男，湖南慈利人，硕士研究生. E-mail:615708949@qq.com
基金资助:
吉林大学基本科研业务费-科学前沿与交叉学科创新项目（200903226）

Immunohistochemical Expression of TNFα and FGF2 in Rat Submandibular Glands Following Irradiation

null

1. Department of Radiology, 2. Department of Orthodontics, 3. Department of Pathology, Jilin University Hospital of Stomatology, Changchun 130021, Jilin Province, China

Online:2015-08-28 Published:2015-12-01

摘要/Abstract

摘要： 目的：观察照射后大鼠颌下腺TNFα和FGF2的表达变化，探讨放疗后唾液腺纤维化的可能分子机制。方法：19只大鼠随机分为对照组（只麻醉不照射）、照射组。照射组用15 Gy的X射线进行颌下腺区局部照射1次，照射后3 d和30 d处死大鼠取颌下腺，4%多聚甲醛固定、石蜡包埋、切片，免疫组化染色观察颌下腺TNFα和FGF2表达的变化。结果：照射后3 d和30 d大鼠颌下腺TNFα和FGF2表达均升高，30 d组高于3 d组。结论：照射后唾液腺纤维化可能与TNFα和FGF2表达升高有关。

关键词: 电离辐射； , 口干症； , TNFα； , FGF2； , 纤维化

Abstract: Objective: The aim of this study was to observe the expression changes of tumor necrosis factor α (TNFα) and fibroblast growth factor 2 (FGF2) in rat submandibular glands（rSMG） after irradiation. Methods: 19 male wistar rats were randomly divided into three groups: group A is no IR group (control group), group B is IR 3-day group, and group C is IR 30-day group. The rats submandibular area were radiated with a single dose of 15 Gy. The animals were anesthetized and sacrificed. rSMG were removed and processed by immunohistochemistry for the detection of TNFα and FGF2. Results: As compared to the control group, TNFα and FGF2 expression were more intensive in IR 3-day group, and most intensive in IR 30-day group. Conclusion: Irradiation induced an increase of the expression of TNFα and FGF2 in rat submandibular glands.

Key words: irradiation, xerostomia, TNFα, FGF2, fibrosis

中图分类号:

朱红华1，鞠昊1，公柏娟2，李志民1，童辉燕1，马珊珊1，孙宏晨3. 电离辐射对大鼠颌下腺TNFα和FGF2表达的影响[J]. 《口腔颌面外科杂志》, 2015, 25(4): 253-.

ZHU Hong-hua1, JU Hao1, GONG Bai-juan2, LI Zhi-min1,TONG Hui-yan1, MAShan-shan1, SUN Hong-chen3. Immunohistochemical Expression of TNFα and FGF2 in Rat Submandibular Glands Following Irradiation[J]. 《Journal of Oral and Maxillofacial Surgery》, 2015, 25(4): 253-.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2015/V25/I4/253

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电离辐射对大鼠颌下腺TNFα和FGF2表达的影响

Immunohistochemical Expression of TNFα and FGF2 in Rat Submandibular Glands Following Irradiation

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电离辐射对大鼠颌下腺TNFα和FGF2表达的影响

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