摘要： 目的：通过水热法改善钛表面结构并加载钙离子，为提高钛种植体早期成骨能力提供实验依据。方法：选用机械加工纯钛片作为对照组；两步法水热处理组，先通过NaOH水溶液水热处理后，以低浓度CaO水溶液水热处理；一步法水热处理组，在高浓度CaO水溶液中180 ℃水热处理24 h。通过SEM、XPS和测量水接触角等测试其表面性能，并通过体外实验检测其对成骨细胞活性的影响。结果：两步法水热组和一步法水热组经处理表面分别形成载钙纳米结构（nm/Ca-Ti）和载钙微米结构（μm/Ca-Ti），且nm/Ca-Ti组经水热处理后亲水性显著提高。经过水热改性后的钛片表面比对照组更有利于细胞的黏附和增殖，且细胞边缘有伪足伸出；在细胞成骨活性方面，载钙纳米结构更具优势。成骨相关基因：Runx2、骨桥蛋白（OPN）和骨钙素（OCN）的表达量显著高于载钙微米组和对照组。结论：经过水热改性形成的载钙纳米结构的钛表面有利于成骨细胞的黏附与增殖，并能有效促进其成骨分化。

关键词: 钛种植体, 水热法, 钙离子, 微纳结构, 骨整合

Abstract: Objective: To study whether the modification of titanium surface with Micro/Nano Structure and Calcium Ions are associated with an early enhanced osseointegration. Methods: We chose commercially pure Ti discs (10 mm×10 mm×2 mm3) with machined surface as control group (control-Ti). Samples in one-step group(μm/Ca-Ti) were hydrothermally treated in high concentration CaO solution at 180℃ for 24h. Samples in two-step group (nm/Ca-Ti), were hydrothermally treated in NaOH solution firstly, then hydrothermally treated in low concentration CaO solution. Surface characteristics were analyzed by SEM, XPS and water contact angle measurement. In vitro experiments were underwent to evaluate bioactivities of different specimens. Results: Nanostructure with Ca2+ and microstructure with Ca2+ were formed on the surface of nm/Ca-Ti and μm/Ca-Ti respectively. Surface hydrophilicity of nm/Ca-Ti was significantly improved after hydrothermal treatment. MC3T3-E1 osteoblast-like cells on treated samples showed much higher bioactivity including cells adhesion and proliferation, and pseudopods of cells were observed on these surface under SEM. Moreover, osteogenesis related genes, including Runx2, osteopontin (OPN) and osteocalcin (OCN), exhibited significantly higher on the surface of nm/Ca-Ti than both μm/Ca-Ti and control-Ti. Conclusion: Nanostructure with Ca2+ on the surface of titanium formed by hydrothermal treatment was confirmed to be beneficial to osteoblast attachment and proliferation, and enhancing cells osteogenic differentiation markedly.

Key words: titanium implant, hydrothermal treatment, calcium ions, micro/nano-structure, osseointegration

中图分类号: 

• R782.4