摘要： 目的：研究牙周膜中的Gli1阳性（Gli1+）细胞的成骨及成牙骨质能力。方法：通过构建特异性表达Gli1的转基因小鼠，观察Gli1阳性细胞在牙周组织中的分布情况，应用免疫荧光染色观察Gli1阳性细胞与runt相关转录因子2（runt-related transcription factor 2，Runx2）和牙骨质附着蛋白（cementum attachment protein，CAP）的共定位情况。结果：成功构建特异性表达Gli1的双基因型小鼠，并观察到Gli1阳性细胞在牙周膜中广泛表达，且大部分均表达Runx2和CAP。结论：Gli1阳性细胞在牙周膜中广泛表达，可能参与了牙周组织的成骨和成牙骨质等生理性改建过程。

关键词: Gli1阳性细胞, 干细胞, mT/mG小鼠, 牙周组织

Abstract: Objective: To investigate the osteogenesis and cementogenic capacities of Gli1+ cells in periodontal membrane. Methods: This study utilized Gli1-CreERT2 mice with B6.129(Cg)-Gt(ROSA)26Sortm4(ACTB-tdTomato,-EGFP)Luo/J mice to generate Gli1-mT/mG mice which specific label Gli1+ cells after tamoxifen treatment. Furthermore, immunofluorescent staining was applied to detect the co-localization of Gli1+ cells with Runx2 (runt-related transcription factor 2) or CAP (cementum attachment protein). Results: This study successfully established the Gli1-mT/mG mice with EGFP protein expression in Gli1 positive cells. In addition, Gli1+ cells were widely expressed in periodontal tissue, and most of them expressed Runx2 and CAP protein. Conclusions: Gli1+ cells were widely expressed in periodontal tissue and probably participated in the process of periodontal physiologic remodeling.

Key words: Gli1+ cells, stem cells, mT/mG mice, periodontal tissue

中图分类号: 

• R780.2

• R329.2