Abstract:

Objective: To investigate the effect of local injection of desferrioxamine (DFO) on the bone remodeling of alveolar bone through the regulation of osteoclasts by hypoxia inducible factor-1α (HIF-1α). Methods: DFO was injected locally into the alveolar bone of C57 mice; changes in alveolar bone volume were observed by micro-CT and histological staining; osteoclastogenesis was observed by immunofluorescence and tartrate-resistant acid phosphase (TRAP) staining; the effects of DFO on the differentiation of Raw264.7 to osteoclasts via HIF-1α were observed in vitro by TRAP staining and immunofluorescence. Results: Micro-CT and histological staining showed that local bone density decreased and bone volume decreased after local injection of DFO; histological staining revealed that the number of local osteoclasts increased and HIF-1α expression increased in the DFO injection group compared with the control group; in vitro cell culture revealed that DFO promoted the expression of HIF-1α in Raw264.7 cells and its differentiation to osteoclasts. Conclusion: Local injection of DFO can promote bone remodeling of alveolar bone and differentiation of Raw264.7 into osteoclasts.

Key words: osteoclasts, hypoxia-inducible factor-1α, alveolar bone

摘要：

目的： 探究局部注射去铁胺（desferrioxamine，DFO）通过低氧诱导因子-1α（hypoxia inducible factor-1α，HIF-1α）调控破骨细胞对牙槽骨骨改建的影响。方法： 对C57小鼠局部牙槽骨注射DFO，通过micro-CT及组织学染色法观察牙槽骨骨量的变化；通过免疫荧光及抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphase，TRAP)染色观察破骨细胞生成，之后用TRAP、免疫荧光等方法观察体外DFO通过HIF-1α对Raw264.7向破骨细胞分化的影响。结果： Micro-CT及组织学染色显示，DFO局部注射后局部骨密度降低、骨量减少；组织学染色发现，相比对照组，DFO注射组局部破骨细胞数量增多、HIF-1α表达增加；体外细胞培养发现DFO可促进Raw264.7细胞中HIF-1α的表达及其向破骨细胞分化。结论： DFO局部注射可促进牙槽骨骨改建及Raw264.7向破骨细胞分化。

关键词: 破骨细胞, 低氧诱导因子-1α, 牙槽骨

CLC Number: 

• R782