《Journal of Oral and Maxillofacial Surgery》 ›› 2024, Vol. 34 ›› Issue (2): 87-93. doi: 10.12439/kqhm.1005-4979.2024.02.002

• Basic Scientific Study • Previous Articles     Next Articles

Local injection of DFO on bone remodeling of alveolar bone: An experimental study in the mouse

ZHANG Qian(), KANG Feiwu()   

  1. Shanghai Engineering Research Center of Tooth Restoration and Regeneration & Tongji Research Institute of Stomatology & Department of Oral and Maxillofacial Surgery, Stomatological Hospital and Dental School, Tongji University, Shanghai 200072, China
  • Received:2022-11-09 Accepted:2022-12-09 Online:2024-04-28 Published:2024-04-29

去铁胺局部注射对小鼠牙槽骨骨改建的实验研究

张倩(), 康非吾()   

  1. 同济大学附属口腔医院口腔颌面外科,同济大学口腔医学院,上海牙组织修复与再生工程技术研究中心,同济大学口腔医学研究所,上海 200072
  • 通讯作者: 康非吾,教授. E-mail: kfw@tongji.edu.cn
  • 作者简介:
    张倩,硕士研究生. E-mail:
  • 基金资助:
    国家自然科学基金(82271013)

Abstract:

Objective: To investigate the effect of local injection of desferrioxamine (DFO) on the bone remodeling of alveolar bone through the regulation of osteoclasts by hypoxia inducible factor-1α (HIF-1α). Methods: DFO was injected locally into the alveolar bone of C57 mice; changes in alveolar bone volume were observed by micro-CT and histological staining; osteoclastogenesis was observed by immunofluorescence and tartrate-resistant acid phosphase (TRAP) staining; the effects of DFO on the differentiation of Raw264.7 to osteoclasts via HIF-1α were observed in vitro by TRAP staining and immunofluorescence. Results: Micro-CT and histological staining showed that local bone density decreased and bone volume decreased after local injection of DFO; histological staining revealed that the number of local osteoclasts increased and HIF-1α expression increased in the DFO injection group compared with the control group; in vitro cell culture revealed that DFO promoted the expression of HIF-1α in Raw264.7 cells and its differentiation to osteoclasts. Conclusion: Local injection of DFO can promote bone remodeling of alveolar bone and differentiation of Raw264.7 into osteoclasts.

Key words: osteoclasts, hypoxia-inducible factor-1α, alveolar bone

摘要:

目的: 探究局部注射去铁胺(desferrioxamine,DFO)通过低氧诱导因子-1α(hypoxia inducible factor-1α,HIF-1α)调控破骨细胞对牙槽骨骨改建的影响。方法: 对C57小鼠局部牙槽骨注射DFO,通过micro-CT及组织学染色法观察牙槽骨骨量的变化;通过免疫荧光及抗酒石酸酸性磷酸酶(tartrate-resistant acid phosphase,TRAP)染色观察破骨细胞生成,之后用TRAP、免疫荧光等方法观察体外DFO通过HIF-1α对Raw264.7向破骨细胞分化的影响。结果: Micro-CT及组织学染色显示,DFO局部注射后局部骨密度降低、骨量减少;组织学染色发现,相比对照组,DFO注射组局部破骨细胞数量增多、HIF-1α表达增加;体外细胞培养发现DFO可促进Raw264.7细胞中HIF-1α的表达及其向破骨细胞分化。结论: DFO局部注射可促进牙槽骨骨改建及Raw264.7向破骨细胞分化。

关键词: 破骨细胞, 低氧诱导因子-1α, 牙槽骨

CLC Number: