Changes of masticatory force affects condyle cartilage development and primary ciliary gene expression in mice

doi:10.12439/kqhm.1005-4979.2024.05.002

Abstract

Abstract:

Objective: To explore the changes in condyle cartilage development and primary ciliary gene expression after changes in masticatory force. Methods: Three-week-old C57BL/6J male mice were divided into 3 groups, and they were fed with normal diet (ND) for 2 weeks, soft diet (SD) for 2 weeks, and fed with 1 week of soft diet than 1 week of re-normal diet (RND). After collecting the samples, safranin O-fast green staining, toluidine blue staining, Masson staining, alkaline phosphatase (ALP) staining, and collagen typeⅡ(COL2) immunofluorescence staining were performed to observe changes in the condylar cartilage and subchondral bone. Primary ciliary gene expression was detected by real-time quantitative polymerase chain reaction (RT-qPCR). Results: Compared with the ND group, the SD group has thinner condyle cartilage, less extracellular matrix, and subchondral bone structure has changed. The primary cilia gene expression (Ift88, Ift140, Ptch1, Smo, Gli1) was decreased. Compared with the SD group, the above changes recovered in the RND group. Conclusion: Changes of masticatory force during growth will affect the development of condyle cartilage and primary ciliary gene expression in mice.

Key words:

condyle , cartilage, , masticatory , force, , primary , cilia

摘要：

目的：探究咀嚼力改变后，髁突软骨发育及初级纤毛基因表达的变化。方法：取 3 周龄雄性 C57BL/6J 小鼠，将其随机分为 3 组，分别给予普通饲料（normal diet，ND）和软食（soft diet，SD）喂养 2 周，以及软食 1 周后恢复普通饲料喂养 1 周（re-normal diet，RND）。行番红 O-固绿、甲苯胺蓝、Masson 三色、碱性磷酸酶（alkaline phosphatase，ALP）、Ⅱ型胶原蛋白（collagen typeⅡ，COL2）免疫荧光染色，观察髁突软骨及软骨下骨的变化情况；实时定量聚合酶链反应（real-time quantitative polymerase chain reaction，RT-qPCR）检测初级纤毛相关基因Ift88、Ift140、Ptch1、Smo、Gli1的表达。结果：相较于 ND 组，SD 组小鼠髁突软骨厚度降低，基质分泌减少，软骨下骨结构改变，初级纤毛相关基因 Ift88、Ift140、Ptch1、Smo、Gli1 的表达有下降趋势；相较于 SD 组，RND 组上述变化有所恢复。结论：生长发育时期，咀嚼力变化可干扰髁突软骨的发育，同时影响初级纤毛相关基因表达。

关键词:

髁突软骨, 咀嚼力, 初级纤毛

CLC Number:

R782

HU Ziyue, ZHANG Shuai, SUN Yao.

Changes of masticatory force affects condyle cartilage development and primary ciliary gene expression in mice

[J]. 《Journal of Oral and Maxillofacial Surgery》, 2024, 34(5): 336-341.

扈梓悦, 张帅, 孙瑶.

咀嚼力变化影响小鼠髁突发育及初级纤毛基因表达

[J]. 《口腔颌面外科杂志》, 2024, 34(5): 336-341.

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URL: https://journal06.magtech.org.cn/Jweb_joms/EN/10.12439/kqhm.1005-4979.2024.05.002

https://journal06.magtech.org.cn/Jweb_joms/EN/Y2024/V34/I5/336

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