《Journal of Oral and Maxillofacial Surgery》 ›› 2012, Vol. 22 ›› Issue (5): 318-322. doi: 10.3969/j.issn.1005-4979.2012.05.003

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Study on the Interaction of Periodontal Ligament Cells and Bone Marrow Stromal Cells in vitro

LIU Shu, LIU Hong-wei   

  1. School of Stomatology, Tongji University, Shanghai 200072, China
  • Online:2012-10-28 Published:2013-01-02

犬牙周膜细胞和骨髓基质细胞相互作用的体外实验研究

刘曙, 刘宏伟   

  1. 同济大学口腔医学院,上海 200072
  • 通讯作者: 刘宏伟,教授. E-mail: hwliu@tongji.edu.cn
  • 作者简介:刘曙 (1978—) ,女,江苏人,硕士研究生. E-mail: anniechongchongls@yahoo.com.cn
  • 基金资助:

    国家自然科学基金资助项目(30772418)

Abstract: Objective: This study was designed to investigate whether the interaction of periodontal ligament cells (PDLCs) and bone marrow stromal cells (BMSCs) is related to better periodontal tissue regeneration. Methods: PDLCs and BMSCs were co-cultured by Transwell chambers. The co-cultured PDLCs and BMSCs were examined by MTT applying 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide and Real-time PCR  assays. Results: PDLCs and BMSCs manifested multilineage differentiation potential. When co-cultured, proliferation rates of PDLCs and BMSCs raised. Gene expressions of COLI, ALP, BSP, OCN, RUNX2 were upregulated. Conclusion: It may be better to use PDLCs and BMSCs together for periodontal tissue regeneration.

Key words: periodontal ligament cells, bone marrow stromal cells, coculture, dog

摘要: 目的:研究牙周膜细胞(PDLCs)和骨髓基质细胞(BMSCs)共培养是否更利于牙周组织再生。方法:用组织块法培养PDLCs,用全血贴壁法培养BMSCs,检测它们的成骨、成脂诱导分化能力。用Transwell小室共培养这两种细胞,通过3-(4,5-二甲基噻唑-2-2,5-二苯基四氮唑溴盐(MTT)和实时荧光定量PCR(Real-time PCR)检测共培养后这两种细胞的增殖能力及部分基因的表达变化。结果:PDLCs和BMSCs具有多向分化潜能。共培养能促进它们各自增殖,上调Ⅰ型胶原(COLI)、碱性磷酸酶(ALP)、骨涎蛋白(BSP)、骨钙素(OCN)、核心结合因子2(RUNX2)的表达。结论:PDLCs和BMSCs共同运用作为种子细胞可能更有利于牙周组织再生。

关键词: 牙周膜细胞, 骨髓基质细胞, 共培养,

CLC Number: