Abstract: Objective: This study was designed to investigate whether the interaction of periodontal ligament cells (PDLCs) and bone marrow stromal cells (BMSCs) is related to better periodontal tissue regeneration. Methods: PDLCs and BMSCs were co-cultured by Transwell chambers. The co-cultured PDLCs and BMSCs were examined by MTT applying 3-(4,5-dimethylthiazol-2-yl-2,5-diphenyltetrazolium bromide and Real-time PCR  assays. Results: PDLCs and BMSCs manifested multilineage differentiation potential. When co-cultured, proliferation rates of PDLCs and BMSCs raised. Gene expressions of COLI, ALP, BSP, OCN, RUNX2 were upregulated. Conclusion: It may be better to use PDLCs and BMSCs together for periodontal tissue regeneration.

Key words: periodontal ligament cells, bone marrow stromal cells, coculture, dog

摘要： 目的：研究牙周膜细胞（PDLCs）和骨髓基质细胞（BMSCs）共培养是否更利于牙周组织再生。方法：用组织块法培养PDLCs，用全血贴壁法培养BMSCs，检测它们的成骨、成脂诱导分化能力。用Transwell小室共培养这两种细胞，通过3-(4,5-二甲基噻唑-2-2,5-二苯基四氮唑溴盐（MTT）和实时荧光定量PCR（Real-time PCR）检测共培养后这两种细胞的增殖能力及部分基因的表达变化。结果：PDLCs和BMSCs具有多向分化潜能。共培养能促进它们各自增殖，上调Ⅰ型胶原（COLI）、碱性磷酸酶（ALP）、骨涎蛋白（BSP）、骨钙素（OCN）、核心结合因子2（RUNX2）的表达。结论：PDLCs和BMSCs共同运用作为种子细胞可能更有利于牙周组织再生。

关键词: 牙周膜细胞, 骨髓基质细胞, 共培养, 犬

CLC Number: 

• R780.2

• Q813.11