Virus Vector-mediated IL-24 Induces Apoptosis in Drug-resistant Oral Squamous Cell Carcinoma KBV Cells

doi:10.3969/j.issn.1005-4979.2015.04.002

Abstract

Abstract: To study the apoptosis-inducing effect of hybrid virus vector-mediated IL-24 gene on oral squamous cell carcinoma (OSCC) cell line and drug-resistant cell line. Methods: In order to identify OSCC cell line KB and the drug resistant counterparts cell lines KBV, cell viability was measured by MTT assay. Fluorescence microscopy and RT-PCR experiments were used to detect the gene expression, AdLTR2EF1α-EGFP or AdLTR2EF1α-IL-24 were used to treat the KB cells in order to obtain the optimal drug concentration. Human keratinocyte HaCat cells were used as control cells. AdLTR2EF1α-IL-24 and AdLTR2EF1α-vec transfected the KB ,KBV, and HaCat cells in a 200 μM substrate solution, and incubated at 37℃ for 1, 3, 5 and 7days respectively. Formation of cells in the sub-G1 phase was indicative of apoptotic cells. Cell viability and apoptosis rate were measured by the MTT assay, flow cytometry. Cells morphology were observed by transmission electron microscope. Results: Vincristine resistance in KBV cells was significantly higher than KB cells. The expression of IL-24 was detected in both transfected KB cells and KBV cells. IL-24 could inhibit the growth of KB cells and KBV cells, while no growth inhibition in HaCaT cells. Overexpression of IL-24 could induce apoptosis in KB and KBV cells, but there was no similar effect in HaCaT cells. Conclusion: Hybrid virus-mediated IL-24 had strong cytotoxicity and induction apoptosis effects on OSCC drug-resistant KBV cell line, and had no side effects on normal cells, which may develop a new way to treat oral drug resistant cancer.

Key words: interleukin-24, oral squamous cell carcinoma, drug resistance, apoptosis

摘要： 目的：研究杂合病毒介导的IL-24基因对口腔鳞状细胞癌耐药细胞的凋亡诱导作用，探讨其治疗耐药口腔癌的可能性。方法：通过MTT细胞活力测定鉴定口腔鳞状细胞癌细胞株KB和其耐药细胞株KBV。利用杂合病毒AdLTR2EF1α搭载EGFP和IL-24转染KB细胞，确定最佳转染浓度，荧光倒置显微镜及RT-PCR鉴定EGFP或IL-24基因表达。以人永生化上皮细胞HaCaT细胞作为对照，使用AdLTR2EF1α-IL-24转染KB、KBV及HaCaT细胞后，MMT法检测细胞活力， AnnexinV/PI双染法检测细胞凋亡，透射电镜观察细胞凋亡形态。结果：KBV细胞对长春新碱的耐受性明显高于KB细胞。AdLTR2EF1α-IL-24搭载的IL-24基因可以在KB及KBV细胞内过表达。IL-24基因在KB和KBV细胞中的过表达，有明显抑制细胞生长和促凋亡作用，而在HaCaT细胞中没有类似的作用。结论：杂合病毒介导的IL-24对口腔鳞状细胞癌耐药细胞有较强的细胞毒性和诱导凋亡作用，并且对正常组织细胞没有影响。具有治疗耐药口腔癌的潜在价值。

关键词: IL-24； , 口腔鳞癌； , 耐药； , 凋亡

CLC Number:

R782
R739.8

YANG De-zhao1, WANG Dan-dan2, MENG Xiang1, YANG Shuai1, SUN Hong-chen2, LI Ji-chen1. Virus Vector-mediated IL-24 Induces Apoptosis in Drug-resistant Oral Squamous Cell Carcinoma KBV Cells[J]. 《Journal of Oral and Maxillofacial Surgery》, 2015, 25(4): 246-.

杨德钊1，王丹丹2，孟祥1，杨帅1，孙宏晨2，李吉辰1. 病毒介导IL-24基因对口腔鳞癌耐药细胞株凋亡的影响[J]. 《口腔颌面外科杂志》, 2015, 25(4): 246-.

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URL: https://journal06.magtech.org.cn/Jweb_joms/EN/10.3969/j.issn.1005-4979.2015.04.002

https://journal06.magtech.org.cn/Jweb_joms/EN/Y2015/V25/I4/246

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