《Journal of Oral and Maxillofacial Surgery》 ›› 2020, Vol. 30 ›› Issue (4): 206-210. doi: 10.3969/j.issn.1005-4979.2020.04.003

• Basic Scientific Study • Previous Articles     Next Articles

The Effect and Mechanism of Theaflavin-3, 3′-digallate on Osteoporosis: A Preliminary Study

AI Zexin1(), LI Jia2, WU Yangou1, YU Miao1, LI Shengjiao1()   

  1. 1 Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Tongji University, Shanghai 200072, China
    2 Department of Prosthodontics, Shanghai 200072, China
  • Received:2019-12-04 Revised:2020-01-20 Online:2020-08-28 Published:2020-08-21

茶黄素-3,3′-双没食子酸酯对骨质疏松的作用及机制初探

艾泽馨1(), 李家2, 吴洋欧1, 于淼1, 李生娇1()   

  1. 1 上海牙组织修复与再生工程技术研究中心,同济大学口腔医学院·同济大学附属口腔医院口腔颌面外科教研室,上海 200072
    2 口腔修复教研室,上海 200072
  • 通讯作者: 李生娇,副教授. E-mail: 07824@tongji.edu.cn
  • 作者简介:

    艾泽馨(1995―),女,内蒙古人,硕士研究生. E-mail:

  • 基金资助:
    上海市科学技术委员会项目(19140904800); 上海市科学技术委员会项目(16ZR1439700)

Abstract:

Objective: To investigate the effect of theaflavin-3, 3′-digallate(TF3) on osteoporosis and its mechanism. Methods: An osteoporosis model in mice was established by ovariectomy(OVX), and TF3 was injected at a gradient concentration (1 mg/kg, 10 mg/kg). After 3 months, mice femurs were taken for Micro-CT analysis. H2O2 was used to establish MC3T3-E1 cells oxidative stress model in vitro. 0, 1, and 10 μmol/L TF3 were added to detect the effects of TF3 on cell activity, the level of intracellular reactive oxygen species (ROS), and the expression of antioxidant genes. Results: Micro-CT analysis showed that TF3 could reduce the volume of trabecular bone loss in osteoporosis, and the degree of bone loss manifested a positive correlation with the increase of the dose of TF3. Further in vitro experiments showed that the activity of MC3T3-E1 cells significantly decreased and the ROS content significantly increased after the addition of H2O2. The addition of TF3 significantly reduced the ROS level and increased the expression level of antioxidant gene Nrf2 and its downstream HO-1 and CAT, and the effect became more obvious with the increase of TF3 concentration. Conclusion: TF3 can reduce the degree of bone loss caused by OVX, which may be related to the protective effect of TF3 on cellular oxidative damage.

Key words: theaflavin-3, 3′-digallate (TF3), MC3T3-E1 cells, oxidative stress, osteoporosis

摘要:

目的:探究茶黄素-3, 3′-双没食子酸酯(theaflavin-3, 3′-digallate,TF3)对骨质疏松的作用及其机制。方法:建立小鼠去卵巢(ovariectomy,OVX)骨质疏松模型,注射剂量为1 mg/kg和10 mg/kg的TF3,3个月后取股骨进行微型CT(Micro-CT)分析。体外应用过氧化氢(H2O2)建立MC3T3-E1细胞氧化应激模型,并分别加入0、1、10 μmol/L 的TF3,检测TF3对细胞活性、细胞内活性氧(reactive oxygen species,ROS)含量和抗氧化基因表达的影响。结果:Micro-CT分析结果表明,TF3能降低骨质疏松的骨小梁丢失程度,且随TF3的剂量增加,效果越明显。进一步的体外实验结果表明,加入H2O2后,MC3T3-E1的细胞活性明显降低,ROS含量明显增加;而同时加入TF3后, ROS水平明显下降,抗氧化基因Nrf2及其下游的HO-1、CAT表达水平增加,且随TF3浓度增加,效果越明显。结论:TF3可以降低OVX引起的骨丢失程度,其作用可能与TF3具有细胞氧化损伤的保护作用有关。

关键词: 茶黄素-3, 3′-双没食子酸酯, MC3T3-E1细胞, 氧化应激, 骨质疏松

CLC Number: