《Journal of Oral and Maxillofacial Surgery》 ›› 2021, Vol. 31 ›› Issue (6): 335-341. doi: 10.3969/j.issn.1005-4979.2021.06.001

• Basic Scientific Study •     Next Articles

Distribution characteristics of K14 positive cells during the injury and regeneration of the submandibular gland

ZHOU Ji(), ZHANG Xueming, GENG Jiangyu, NIU Pingping, SUN Yao()   

  1. Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Oral Implantology, School and Hospital of Stomatology, Tongji University, Shanghai 200072, China
  • Received:2020-12-07 Revised:2021-04-02 Online:2021-12-28 Published:2021-12-30

K14阳性细胞在下颌下腺损伤修复中的分布特征

周济(), 张雪明, 耿江予, 牛萍萍, 孙瑶()   

  1. 上海牙组织修复与再生工程技术研究中心,同济大学口腔医学院,同济大学附属口腔医院口腔种植科,上海 200072
  • 通讯作者: 孙 瑶,教授. E-mail:
  • 作者简介:

    周 济(1994—),女,江苏人,硕士. E-mail:

  • 基金资助:
    国家重点研发计划项目(2016YFC1102700); 国家自然科学基金(81822012)

Abstract:

Objective: Cytokeratin 14(K14) is the marker of salivary gland stem/progenitor cells. This study aimed to explore the expression and role of K14+ during the injury and regeneration process of the submandibular gland (SMG). Methods: The main salivary gland ducts of 8-week-old mice were ligated for 14-d and then deligated. The mice were sacrificed at 0-d, 14-d after ligation and 3-d, 28-d, 56-d after deligation. The injury and regeneration of SMG were observed by histological staining, and the proliferation of K14+ cells was observed by immunofluorescence staining. The mRNA expression level and changes of genes related to salivary gland function were detected by real-time PCR. Results: Mouse SMG duct ligation-deligation model was successfully established. The number of K14+ cells gradually decreased over time during the development period of the SMG. The glands showed atrophy and the duct showed dilatation after the main duct was ligated; the number of K14+ cells increased in the early stage of SMG regeneration, and most of them were in a proliferating state; but the expression level of genes related to salivary gland function was low. Conclusion: During the injury and regeneration process of the SMG, the expression of K14+ cells increased in the early stage during regeneration, suggesting that K14+ cells are involved in the regeneration process of the SMG.

Key words: submandibular gland, injury, regeneration, cytokeratin 14

摘要:

目的:角蛋白14(cytokeratin 14, K14)是唾液腺干/祖细胞的标志物,本研究探讨K14+细胞在下颌下腺损伤修复中表达分布的特征。方法:构建8周龄小鼠双侧下颌下腺主导管结扎-开放模型,分别于结扎后0、14 d及开放后3、28、56 d收样,利用组织学染色法评估下颌下腺损伤及再生状况,免疫荧光染色法观察K14+细胞增殖,实时荧光定量PCR检测唾液腺功能相关基因表达的变化。结果:在下颌下腺发育过程中K14+细胞随着年龄增长逐渐减少;成功构建小鼠下颌下腺主导管结扎-开放模型,导管结扎后,腺体萎缩,导管扩张;开放导管后,腺体修复早期K14+细胞数目增多,增殖活跃,但是唾液腺功能相关基因表达较低。结论:在下颌下腺损伤修复早期K14+细胞表达增加,提示K14+细胞参与了下颌下腺损伤修复的过程。

关键词: 下颌下腺, 损伤, 再生, 角蛋白14