《Journal of Oral and Maxillofacial Surgery》 ›› 2021, Vol. 31 ›› Issue (6): 342-348. doi: 10.3969/j.issn.1005-4979.2021.06.002

• Basic Scientific Study • Previous Articles     Next Articles

Construction of mesenchymal stem cell membrane-coated biomimetic nanocarrier and its effect on polarization of macrophages

LI Jialin(), SU Jiansheng()   

  1. Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Department of Prosthodontics, School and Hospital of Stomatology, Tongji University, Shanghai 200072, China
  • Received:2021-02-05 Revised:2021-03-21 Online:2021-12-28 Published:2021-12-30

间充质干细胞膜包被的仿生纳米载体的构建及其对巨噬细胞极化基因表达影响的研究

李家琳(), 苏俭生()   

  1. 上海牙组织修复与再生工程技术研究中心,同济大学口腔医学院,同济大学附属口腔医院口腔修复科,上海 200072
  • 通讯作者: 苏俭生,教授. E-mail:
  • 作者简介:

    李家琳(1994—),女,辽宁人,硕士. E-mail:

  • 基金资助:
    国家自然科学基金(81873715); 国家自然科学基金(81572114); 上海市科学技术委员会项目(18441902100)

Abstract:

Objective: To construct the mesenchymal stem cells(MSCs) membrane-coated, curcumin (Cur)-loaded, biomimetic nanocarrier and evaluate its effects on the expression levels of genes related to macrophage polarization. Methods: A liposome mini-extruder was used to fuse MSCs plasma membranes (PMs) onto Cur-loaded poly(lactic-co-glycolic) (PLGA) nanoparticles to construct the PMs-coated bionic nanocarriers. The morphology of nanocarriers was characterized by transmission electron microscopy (TEM). The drug release pattern and the stability of nanocarriers were investigated in vitro. After co-culturing the nanocarriers with RAW264.7 macrophages, cytotoxicity and the expression levels of macrophage polarization related genes were detected. Results: We successfully constructed the PMs-coated, biomimetic nanocarriers. A core-shell structure could be observed under TEM. The particle size increased from (183.2±0.8) nm to (204.2±3.7) nm after coating. The stability of the material performed well in vitro and 24 h cumulative drug release reached 55.37%. The expression levels of M2 macrophages related genes IL-10, Arg1, CD206 were significantly enhanced in the test group, while the expression of M1 macrophages related genes iNOS and IL-1β decreased. Conclusion: This study successfully constructed the biomimetic nanocarriers coated by MSCs PMs, which can effectively promote the expression of M2 macrophages related genes.

Key words: cell membrane, biomimetic nanocarrier, macrophage

摘要:

目的:构建间充质干细胞(mesenchymal stem cells, MSCs)膜包被的仿生纳米载体,负载姜黄素,探究其对巨噬细胞极化基因表达的影响。方法:利用脂质体挤压器将干细胞膜囊泡与载有姜黄素的聚乳酸-羟基乙酸共聚物[poly (lactic-co-glycolic),PLGA]纳米粒结合,构建MSCs膜包被的仿生纳米载体。应用透射电镜表征形貌。体外考察其稳定性和药物释放规律。将材料与RAW264.7巨噬细胞共培养,探究材料的细胞毒性及巨噬细胞极化相关基因的表达水平。结果:成功构建MSCs膜包被的仿生纳米载体,电镜下见均匀核-壳球形结构。粒径由包裹前(183.2±0.8) nm转变为(204.2±3.7) nm。体外稳定性良好,24 h累积药物释放55.37%。实验组M2型巨噬细胞相关基因IL-10、Arg1、CD206表达升高,M1型巨噬细胞相关基因iNOS、IL-1β降低。结论:本实验成功构建干细胞膜包被的仿生纳米载体,能有效地促进M2型巨噬细胞相关基因表达。

关键词: 细胞膜, 仿生纳米载体, 巨噬细胞