miR-30e-5p Regulates Proliferation and Migration of Oral Squamous Cell Carcinoma

doi:10.3969/j.issn.1005-4979.2018.03.003

Abstract

Abstract: Objective: The aim of this study was to investigate the effects of miR-30e-5p on the proliferation and migration of tumor cells in oral squamous cell carcinoma (OSCC), and to determine its role in OSCC progression. Methods: The expressions of miR-30e-5p in OSCC tissues, adjacent normal tissues, normal oral mucosal epithelial cells and HN6 cells were individually examined by real-time quantitative PCR. The miR-30e-5p mimics, miR-30e-5p inhibitor, mimics NC and inhibitor NC were transfected into HN6 cells, respectively. Transfection efficiency and expression level of miR-30e-5p were detected by quantitative real-time PCR assay. The proliferation assay, plate colony formation assay and wound healing assay were performed to study the effects of miR-30e-5p on cell proliferation and migration. Results: The expressions of miR-30e-5p was down-regulated in OSCC tissues when compared with the paired normal tissues (P<0.05). Similarly, the expressions of miR-30e-5p was down-regulated in HN6 cells when compared with the normal oral mucosal epithelial cells (P<0.05). Overexpression of miR-30e-5p in HN6 cells significantly inhibited the proliferation, colony formation and migration abilities of HN6 cells (P<0.05); while silencing of miR-30e-5p by miR-30e-5p inhibitor in HN6 cells remarkably promote the proliferation, colony formation and migration abilities of HN6 cells (P<0.05). Conclusions: miR-30e-5p was down-regulated in OSCC and the expression of miR-30e-5p was correlated with the proliferation and migration of tumor cells, which indicated that miR-30e-5p may play a vital role as tumor suppressor gene during the development and progression of OSCC.

Key words: miR-30e-5p, oral squamous cell carcinoma, proliferation, migration

摘要： 目的：研究miR-30e-5p对口腔鳞癌细胞增殖和迁移能力的影响，探讨其在口腔鳞癌发生发展中的作用。方法：实时荧光定量PCR检测miR-30e-5p在人口腔鳞癌组织、癌旁组织、正常口腔黏膜上皮细胞和HN6细胞系中的表达水平。应用miR-30e-5p mimics、miR-30e-5p inhibitor、mimics NC、inhibitor NC分别转染HN6细胞，实时荧光定量PCR检测转染后miR-30e-5p的表达变化；采用细胞增殖实验、平板克隆形成实验、划痕实验分别检测miR-30e-5p对HN6细胞生长和迁移的影响。结果：相对于癌旁正常组织，miR-30e-5p在口腔鳞癌组织中的表达显著下调（P<0.05）。相对于正常口腔黏膜上皮细胞，miR-30e-5p在口腔鳞癌组织中的表达显著下调（P<0.05）。转染miR-30e-5p mimics后，HN6细胞的增殖能力、克隆形成能力及迁移能力显著降低，而转染miR-30e-5p inhibitor后，HN6细胞的增殖能力、克隆形成能力及迁移能力则明显升高，差异均具有统计学意义（P<0.05）。结论：miR-30e-5p在口腔鳞癌组织中低表达，与口腔鳞癌细胞的生长和迁移密切相关，提示miR-30e-5p在口腔鳞癌的发生、发展过程中可能起抑癌基因的作用。

关键词: miR-30e-5p, 口腔鳞癌, 增殖, 迁移

CLC Number:

R782.4

ZHU Xue-qin, YE Dong-xia, QIN Xing, CHEN Wan-tao. miR-30e-5p Regulates Proliferation and Migration of Oral Squamous Cell Carcinoma[J]. 《Journal of Oral and Maxillofacial Surgery》, doi: 10.3969/j.issn.1005-4979.2018.03.003.

朱雪琴，叶冬霞，秦星，陈万涛. miR-30e-5p调控口腔鳞癌细胞增殖和迁移的实验研究[J]. 《口腔颌面外科杂志》, doi: 10.3969/j.issn.1005-4979.2018.03.003.

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URL: https://journal06.magtech.org.cn/Jweb_joms/EN/10.3969/j.issn.1005-4979.2018.03.003

https://journal06.magtech.org.cn/Jweb_joms/EN/Y2018/V28/I3/135

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