Construction of the mandible deviation occlusion rat model and micro-CT radiography and histology evaluation of the mandibular condyles

doi:10.3969/j.issn.1005-4979.2021.05.003

Abstract

Abstract:

Objective: To construction a mandible deviated occlusion(MDO) rat model and investigate the changes in the micro-CT images and histology of condyles. Methods: Twelve 6-week-old female SD rats were randomly divided into control group and MDO group (n=6 in each group). The method of MDO model: The left maxillary central incisors and the bilateral mandibular central incisors were bonded with the metal tubed aberrant prosthesis. The maxillary metal tube was designed to have a lingual inclined guide plate in order to achieve normally derived over-jet and over-bite relationship. The mandibular metal tube was designed to have an inclined plate, 45 degrees in mesial-distal direction to guide the mandible to deviate to right side when rat biting. The control group did not do any treatment. The rats in the two groups were fed for 12 weeks, and bilateral mandibular condyles were collected for micro-CT scanning and then taken for histological staining. The data of bilateral condyles in the control group were combined to get the average value. The data from the MDO group were divided into the MDO-left group and the MDO-right group. Results: Micro-CT images showed that the condyles in control group had smooth and intact articular surface. The density of the subchondral bone was normally impact. In contrast, the length and width of the condyles in MDO group were increased in varying degree and trabecular bone loss was obviously absorbed. Three left condyles out of the total twelve condyles in MDO group showed lacunae on the articular surface. Histological staining showed that the condyle chondrocytes in control group had well organized zonal cartilage. While in the MDO-left group, lacunaes of left condyles were filled with cartilage tissue or fibrous tissue. Histomorphometry analysis showed that the cartilage in middle third of the condyle in both MDO-left and MDO-right groups was thicker than that in control group (P<0.05). Unmineralized cartilage in posterior third of MDO-left group was thicker than that in control group (P<0.05). Conclusion: MDO could lead to asymmetrically aberrant remodeling of bone and cartilage in the mandibular condyles, and formation of cartilage projections in the articular surface which displayed on micro-CT images as lacuna of the condylar articular surface.

Key words: temporomandibular joint, trabecular bone resorption, mandible deviation occlusion, cartilage lacunae

摘要：

目的： 构建下颌偏斜大鼠模型，观察该实验性偏颌大鼠髁突显微CT（micro-CT）影像及其组织学变化。方法： 选取12只6周龄雌性SD大鼠，将其随机等量分为对照组和偏颌组，每组6只。偏颌造模方法：将上颌左侧中切牙及下颌双侧中切牙分别粘接金属套筒冠不良修复体，上颌金属套筒冠制备唇向斜面导板，使上下颌切牙有正常方向的覆牙合覆盖关系，下颌金属套筒冠制备与牙长轴呈45°角的近远中向斜面导板，使动物咬合时导板可以引导下颌向右。对照组不做任何处理。2组大鼠饲养12周后取材，对双侧髁突进行micro-CT检测及组织学染色。将对照组双侧髁突测量的数据合并取平均值，将偏颌组分为偏颌组-左侧和偏颌组-右侧，分别与对照组比较。结果： Micro-CT结果显示，对照组大鼠髁突关节面光滑、完整，骨质致密。与对照组髁突相比，偏颌组髁突长度和宽度出现不同程度的增加，但骨小梁有明显的吸收；12个髁突中有3个左侧髁突表面出现陷窝。组织学染色结果显示，对照组大鼠髁突软骨细胞层次分明，而偏颌组大鼠左侧髁突骨性陷窝内有软骨组织或纤维组织充填。组织测量学结果显示，偏颌组双侧大鼠髁突未矿化软骨的中带较对照组有显著增厚（P<0.05），偏颌组左侧髁突未矿化软骨的后带较对照组有明显增厚（P<0.05）。结论： 实验性偏颌可致大鼠双侧髁突骨和软骨出现非对称性异常改建，偏颌对侧髁突表面可出现局部纤维软骨样组织增厚，在影像学上表现为关节面上缺损样陷窝。

关键词: 颞下颌关节, 骨吸收, 偏颌, 软骨陷窝

ZHANG Yuejiao, XU Xiaojie, LIU Qian, ZHAO Yali, LIU Jiguang, WANG Meiqing. Construction of the mandible deviation occlusion rat model and micro-CT radiography and histology evaluation of the mandibular condyles[J]. 《Journal of Oral and Maxillofacial Surgery》, 2021, 31(5): 278-284.

张月姣, 徐小杰, 刘倩, 赵雅莉, 刘继光, 王美青. 偏颌大鼠模型的构建及其髁突CT影像和组织学评价[J]. 《口腔颌面外科杂志》, 2021, 31(5): 278-284.

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URL: https://journal06.magtech.org.cn/Jweb_joms/EN/10.3969/j.issn.1005-4979.2021.05.003

https://journal06.magtech.org.cn/Jweb_joms/EN/Y2021/V31/I5/278

Figures/Tables 7

Figure 1 Representative frontal view and the sketch map of the incisal occlusal relationship in control group and MDO group of rats

Figure 2 The measuring methods of micro-CT images

Figure 3 The cartilage thickness revealed by sections with VK staining and TB staining, the trisections were the anterior, middle and posterior third of the condyle

Figure 4 Comparisons of the parameters measured from the micro-CT images between the control group and the left and right side condyles from the MDO group

Figure 5 The representative micro-CT images in the sagittal sections of the mandibular condyles from the control group and that of the left and right side condyles from the MDO group

Figure 6 Representative images with VK staining, TB staining and HE staining for the sagittal sections of the mandibular condyles from the control group and that of the left and right side condyles from the MDO group

Figure 7 Comparisons of the cartilage body thickness (based on TB staining) and the un-mineralized cartilage thickness (based on VK staining) between the control group and the left and right side condyles from the MDO group

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