Abstract:

Objective: To study the effects of a Chinese traditional medicine icariin (ICA) on the cell activity and osteogenic differentiation of preosteoblasts in mice calvarium (MC3T3-E1).Methods: The optimal intervention concentration of ICA was screened and MC3T3-E1 cells were divided into blank control group and experimental group with different concentrations of ICA. MC3T3-E1 cells were treated with ICA complete medium containing 10^-9, 10^-8, 10^-7, 10^-6 and 10^-5 mol/L. On days 2, 4 and 6, the proliferation of MC3T3-E1 cells was detected by CCK8 kit, and the ALP activity was detected by alkaline phosphatase (ALP) kit on days 3, 6 and 9; MC3T3-E1 cells were treated with 10^-7 mol/L ICA complete medium for 21 days. Alizarin red (AR) staining was used to determine the formation of calcium nodules.Results: Compared with the blank control group, the drug treatment group significantly inhibited the proliferation of MC3T3-E1 cells, but promoted the ALP activity of MC3T3-E1 cells; however, different concentrations of ICA had no significant effect on the formation of calcium nodules in cultured cells.Conclusion: Under our experimental conditions, ICA may inhibit the proliferation of MC3T3-E1 cells and promote osteogenic differentiation of MC3T3-E1 cells.

Key words: icariin, MC3T3-E1 cells, proliferation, osteogenic differentiation

摘要：

目的： 研究传统中药淫羊藿的主要活性成分淫羊藿苷（icariin，ICA）对小鼠颅顶前成骨细胞（preosteoblasts in mice calvarium，MC3T3-E1 )的细胞活性及成骨分化的影响。方法： 筛选出ICA的最佳干预浓度，将MC3T3-E1细胞分为空白对照组及不同浓度的实验组，实验组以含10^-9、10^-8、10^-7、10^-6、10^-5 mol/L的ICA完全培养液分别处理MC3T3-E1细胞。分别于培养2、4、6 d后用CCK8试剂盒检测MC3T3-E1细胞增殖能力；于培养3、6、9 d后用碱性磷酸酶（alkaline phosphatase，ALP）试剂盒检测其ALP活性；根据检测结果，分别用完全培养液、成骨诱导液、含10^-7 mol/L的ICA完全培养液处理MC3T3-E1细胞21 d后，再分别作茜素红染色。结果： 与空白对照组相比，药物处理组明显抑制了MC3T3-E1细胞的增殖，但促进了MC3T3-E1细胞的ALP活性；而不同浓度的ICA培养的细胞对其钙结节的形成影响不明显。结论： 在我们的实验条件下，ICA可能抑制了MC3T3-E1细胞的增殖，促进了MC3T3-E1细胞的成骨分化。

关键词: 淫羊藿苷, 小鼠颅顶前成骨细胞, 增殖, 成骨分化

CLC Number: 

• R782