Abstract:

Objective: To investigate the effect of miR-181a-5p on osteogenic differentiation of human bone marrow mesenchymal stem cells (BMSCs) during bone remodeling after bone fracture. Methods: BMSCs were extracted from the discarded bone of patients who received orthognathic surgery and were divided into control group, miR-181a-5p overexpression group and miR-181a-5p silencing group after transfection. After 14-day osteogenic induction, the formation of mineralized nodules and the expression of alkaline phosphatase (ALP) were analyzed. And the expression of osteogenic genes were analyzed by real-time quantitative polymerase chain reaction(RT-qPCR) after 21 days. Results: Compared with the control group, the miR-181a-5p overexpression group had higher expression level of ALP, higher expression level of osteogenic genes such as collagen type 1 (COL1), runt related transcription factor 2 (Runx2), and more formation of mineralized nodules. The miR-181a-5p silencing group had lower expression level of ALP, osteogenic genes COL1, Runx2, and less formation of mineralized nodules than that of the control group. Conclusion: miR-181a-5p can promote the osteogenic differentiation of BMSCs.

Key words: miR-181a-5p, bone marrow mesenchymal stem cells, osteogenic differentiation

摘要：

目的：探讨骨折后骨改建中miR-181a-5p对人骨髓间充质干细胞（bone marrow mesenchymal stem cells，BMSCs）成骨分化的作用。方法：提取正颌手术患者废弃骨块中的BMSCs，将其转染后分为对照组、miR-181a-5p过表达组、miR-181a-5p沉默组。成骨诱导14 d后，分析各组矿化结节形成、碱性磷酸酶（alkaline phosphatase, ALP）表达。成骨诱导21 d后，实时定量聚合酶链反应（real-time quantitative polymerase chain reaction，RT-qPCR）分析各组成骨相关基因的表达。结果：miR-181a-5p过表达组相比对照组，其ALP表达更高，成骨相关基因Ⅰ型胶原（collagen type 1,COL1）、Runt相关转录因子2（runt related transcription factor 2, Runx2）表达更高，矿化结节形成更多；miR-181a-5p沉默组相比对照组，其ALP表达更低，成骨相关基因COL1、Runx2表达更低，矿化结节形成更少。结论：miR-181a-5p对BMSCs成骨分化具有促进作用。

关键词: miR-181a-5p, 骨髓间充质干细胞, 成骨分化

CLC Number: 

• R782