《口腔颌面外科杂志》 ›› 2012, Vol. 22 ›› Issue (3): 157-162. doi: 10.3969/j.issn.1005-4979.2012.03.002

• 基础研究 • 上一篇    下一篇

Rad50在放射后大鼠涎腺中的表达

陈超梅, 王代友, 林丹, 沈洁, 陆新萍   

  1.  广西医科大学附属口腔医院口腔颌面外科, 广西 南宁 530021
  • 出版日期:2012-06-28 发布日期:2012-08-30
  • 通讯作者: 王代友,教授. E-mail: wangdaiyou@sina.com
  • 作者简介:陈超梅(1985—),女,广西南宁人,硕士研究生. E-mail: mayhotis@163.com
  • 基金资助:

    国家自然科学基金资助项目(30960420);广西医疗卫生重点科研课题(桂卫重2010082)

Differential Expression of Rad50 in Mouse Salivary Gland  Following Irradiation

CHEN Chao-mei,WANG Dai-you,LIN Dan,SHEN Jie,LU Xin-ping   

  1. Department of Oral and Maxillofacial Surgery,Hospital of Stomatology,Guangxi Medical University,Nanning 530021,Guangxi Zhuang Autonomous Region,China
  • Online:2012-06-28 Published:2012-08-30
  • About author:王代友,教授. E-mail: wangdaiyou@sina.com

摘要:   目的: 检测Rad50在放射后大鼠涎腺组织中的表达水平。方法: 选用 60只Wistar大白鼠,随机分成6组,每组10只。分为对照组(未照射)、3 Gy组、6 Gy组、9 Gy组、12 Gy组、15 Gy组。放射组大白鼠用60Co一次性照射后2 h内处死,立即取出其腮腺、下颌下腺组织备用。用电镜观察放射后各组涎腺组织超微结构的变化,用反转录聚合酶链反应 (RT-PCR)检测Rad50基因表达水平的变化,用免疫组织化学法观察其蛋白表达水平的变化。结果: 各放射组涎腺组织中Rad50的表达均高于对照组 (P<0.05),但不同放射剂量的各放射组之间Rad50的表达无明显差异(P > 0.05)。透射电镜下可见大鼠腮腺、下颌下腺细胞超微结构随着放射剂量的增加,发生较明显改变。结论: 放射可引起涎腺组织Rad50表达水平增高,但其表达水平并未随放射剂量的增加而增高,提示Rad50在涎腺放射损伤修复中的表达水平有限,这可能是涎腺放射敏感性机制之一。 

关键词: Rad50, 放射, 腮腺, 下颌下腺,

Abstract: Objective: The present study was designed to examine the expression levels of Rad50 in the salivary gland of Wistar rats after irradiation. Methods: 60 Wistar rats were randomly distributed into 6 groups: five groups  were irradiated for 3,6,9,12,and 15 Gy doses of 60Co γ-ray respectively,  and a sham irradiation group as control. The rats were sacrificed at 2-hour after irradiation. Histopathological changes in the parotid and submandibular glands  were observed by  transmission electron microscopy (TEM). Rad50 mRNA levels were evaluated by RT-PCR. Rad50 protein levels were evaluated by immunohistochemistry method. Statistical analysis were performed by means of SPSS 13.0 software. The experimental data were expressed as x±s; comparisons were made by factorial ANOVA. P<0.05 were considered significant. Results: Expressions of Rad50 mRNA and protein level in  parotid glands and submandibular glands after irradiation were significantly higher than those in normal control salivary glands (P<0.05). The expression level of Rad50 in each irradiated groups appeared differently but did not reach statistical significance (P>0.05). Ultra structures of those irradiated salivary gland cells showed injured variations at some degree in a dose-dependent manner as demonstrated by the transmission electron microscope. Conclusion: γ-ray  radiation may affect expression of Rad50 mRNA and protein levels  in the parotid and submandibular glands, but the expression intensity does not manifested in a radiation dose-dependent manner. Our study demonstrate that the limitation of expression potential of Rad50 in the salivary glands, may be  related to the radiation unsensitivities of the salivary glands.

Key words: Rad50, irradiation, parotid gland, submandibular gland, rat

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