环孢素A致牙龈增生与菌斑、胶原代谢的相关性研究

doi:10.3969/j.issn.1005-4979.2014.01.003

《口腔颌面外科杂志》 ›› 2014, Vol. 24 ›› Issue (1): 10-. doi: 10.3969/j.issn.1005-4979.2014.01.003

环孢素A致牙龈增生与菌斑、胶原代谢的相关性研究

赵婧，高明津，姜蕾，赵云富

第二军医大学附属长征医院口腔科，上海 200003

出版日期:2014-02-28 发布日期:2014-06-27
通讯作者: 赵云富，教授. E-mail:zhaoyf1818@126.com
作者简介:赵婧（1985—），女，黑龙江人，讲师，硕士.E-mail: joanjing1985@126.com
基金资助:
2011年国家自然基金资助项目（81170966）

Oral Care and Collagen Metabolism in the Development of CIGO

ZHAO Jing, GAO Ming-jing, JIANG Lei, ZHAO Yun-fu

Department of Stomatology, Changzheng Hospital, Second Military Medical
University, Shanghai 200003, China

Online:2014-02-28 Published:2014-06-27

摘要/Abstract

摘要： 目的：探讨菌斑存在与否对环孢素A（CsA）所致牙龈增生（CIGO）的影响及其可能的发病机制。方法：SD雄性大鼠40只，实验组、对照组各20只，均拔除左侧上颌磨牙，在同一SD大鼠口内建立有/无菌斑条件下的牙龈模型。实验组每天胃饲含CAS的橄榄油溶液（30 mg/kg），对照组胃饲等量纯橄榄油。定期观察两组大鼠牙龈增生情况，运用免疫组化和RT?鄄PCR方法观察两组大鼠牙龈内CyPA、CD147、MMP?鄄2的表达差异。结果：实验组上颌双侧牙龈增生明显，同一大鼠口内拔牙侧和非拔牙侧牙龈增生程度无显著差异； CyPA和MMP?鄄2的蛋白和mRNA表达水平实验组较对照组均明显减少（P<0.01）；CD147的蛋白质和mRMA表达水平在两组间均无显著差异（P>0.05）；实验组和对照组中有牙侧和无牙侧CyPA、CD147和MMP?鄄2的蛋白质和mRMA表达水平均无显著差异（P>0.05）。结论：即使无菌斑存在，仍有CIGO发生； CyPA与MMP?鄄2表达的下降，可能是牙龈组织中胶原降解减少、牙龈增生的原因之一。

关键词: 环孢素A； , 牙龈增生； , 菌斑； , 基质金属蛋白酶-2； , CD147； , 亲环素A

Abstract: Objective: The aim of the study was to investigate the role of the plaque formation on the development of cyclosporine A induced gingival overgrowth (CIGO). Methods: 40 Sprague-Dawley rats were selected and subjected to all left maxillary molars extraction. All rats were randomly divided into two groups. Rats in the test group (n=20) received cyclosporine A (CsA), which was dissolved in olive oil via gastric feeding (30 mg/kg body weight every day) to establish a rat model of the CIGO. Rats in the control group (n=20) received olive oil only. After 4 weeks, rats were sacrificed and gingival tissues were observed by macroscopic and microscopic methods. The expressions of CyPA, CD147 and MMP-2 in gingival tissues were evaluated by immunohistochemistry and Real-time PCR. Results: In the CsA-treated animals, both sides of the maxillary gingival were found to be enlarged and the body weights reduced. In the control group, expressions of CyPA and MMP-2 at protein and mRNA levels were obviously higher than that in test group(P<0.01). But no significant difference was found in CD147 expression between two groups(P>0.05). Conclusion: Oral care manifested as plaque formation is the relevant factor but not initiating factor of CIGO. The mechanism of CIGO may be concerned with the formation of CsA-CyPA complex and competitive inhibition of the interaction between CyPA and CD147, leading to imbalance of collagen degradation and accumulation, which causes gingival overgrowth.

Key words: cyclosporin A, gingival overgrowth; , plaque, matrix metalloproteinase-2; , CD147; , cyclophilin A

中图分类号:

R781.4

赵婧，高明津，姜蕾，赵云富. 环孢素A致牙龈增生与菌斑、胶原代谢的相关性研究[J]. 《口腔颌面外科杂志》, 2014, 24(1): 10-.

ZHAO Jing, GAO Ming-jing, JIANG Lei, ZHAO Yun-fu. Oral Care and Collagen Metabolism in the Development of CIGO[J]. 《Journal of Oral and Maxillofacial Surgery》, 2014, 24(1): 10-.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2014/V24/I1/10

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