珍珠粉、rhBMP-2及其复合物在骨组织修复中的应用研究进展

doi:10.3969/j.issn.1005-4979.2018.03.011

摘要/Abstract

摘要： 探寻适用于临床的理想骨修复材料一直是生物工程材料研究的热点之一。珍珠粉作为天然的有机材料，其成分以及生物力学作用与骨组织相接近，将其作为研究对象应用于骨修复材料有很好的前景。重组人骨形态发生蛋白2（recombinant human bone morphogenetic protein 2，rhBMP-2）是目前已知的促进骨折愈合的生长因子，在骨修复材料中的应用也相当广泛。本文就珍珠粉、rhBMP-2 以及其复合物目前在骨组织修复中的应用研究进展进行系统综述。同时还分析了材料所存在的问题，展望了该复合材料的应用前景。

关键词: 珍珠粉, rhBMP-2, 组织工程, 植骨材料

Abstract: Pearl powder has been one of the hotspots in the research of bioengineering materials to explore the ideal bone repair materials suitable for clinical application. Recombinant human bone morphogenetic protein 2(rhBMP-2) is a known growth factor to promote fracture healing, and is widely used in bone tissue engineering. This article aims to summarize the potentials as well as the problems of the pearl powder , rhBMP-2 and their complex in bone engineering.

Key words: pearl powder, rhBMP-2, tissue engineering, bone substitute

中图分类号:

R782.4

李娜，陈灼庚（综述），徐普（校审）. 珍珠粉、rhBMP-2及其复合物在骨组织修复中的应用研究进展[J]. 《口腔颌面外科杂志》, doi: 10.3969/j.issn.1005-4979.2018.03.011.

LI Na, CHEN Zhuo-geng, XU Pu. Recent Advance on the Application of Pearl Powder，rhBMP-2 and their Complex in Bone Engineering[J]. 《Journal of Oral and Maxillofacial Surgery》, doi: 10.3969/j.issn.1005-4979.2018.03.011.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2018/V28/I3/176

参考文献

[1] Martina M, Subramanyam G, Weaver JC, et al. Developing macroporous bicontinuous materials as scaffolds for tissue engineering[J]. Biomaterials, 2005, 26(28):5609-5616.

[2] Zakaria SM, Zein SH S, Othman MR, et al. Nanophase hydroxyapatite as a biomaterial in advanced hard tisue enginering:a review[J].Tissue eng Part B Rev, 19(5):431-441.

[3] 曹谊林. 组织工程学理论与实践[M]. 上海: 上海科学技术出版社，2004:3-8

[4] Nukavarapu SP, Kumbar SG, Brown JL, et al. Polyphosphazene/nano-Hydroxyapatite composite microsphere scaffolds for bone tissue engineering[J]. Biomacromolecules, 2008, 9:(7):1818-1825.

[5] 毛秋华, 徐普, 王彬娉,等. 纳米级淡水珍珠粉的制备研究[J]. 口腔医学研究. 2016, 32(12):1244-1247.

[6] Lieberman JR, Daluiski A, Einhom TA, et al. The role of growth factors in the repair of bone biology and clinical applications[J]. J Bone Joint Surg Am, 2002,84-A(6):1032-1044.

[7] Chappuis V, Gamer L, Cox K, et al. Periosteal BMP2 activity drives bone graft healing[J]. Bone, 2012, 51(4):800-809.

[8] Mussano F, Ciccone G, Ceccarelli M, et al. Bone morphogenetic proteins and bone defects: a systematic review[J]. Spine (Phila Pa 1976), 2007, 32(7):824-830.

[9] Alakpa EV, Burgess KEV, Chung P, et al. Nacre topography produces higher crystallinity in bone than chemically induced osteogenesis[J]. ACS nano, 2017, 11(7):6717-6727.

[10] Ma MG, Dong YY, Fu LH, et al.Cellulose/CaCO3 nanocomposites: microwave ionic liquid synthesis, characterization, and biological activity[J]. Carbohydr polym, 2013, 92(2):1669-1676.

[11] Mouriès LP, Almeida MJ, Milet C, et al. Bioactivity of nacre water-soluble organic matrix from the bivalve mollusk pinctada maxima in three mammalian cell types: fibroblasts, bone marrow stromal cells and osteoblasts[J]. Comp Biochem Physiol B Biochem Mol Biol, 2002,132(1):217-229.

[12] Lamghari M, Almeida MJ, Berland S, et al. Stimulation of bone marrow cells and bone formation by nacre: in vivo and in vitro studies[J]. Bone, 1999, 25(2 Suppl):S91-S94.

[13] Flausse A, Henrionnet C, Dossot M. Osteogenic differentiation of human bone marrow mesenchymal stem cells in hydrogel containing nacre powder[J]. J Biomed Mater Res A, 2013, 101(11):3211-3218.

[14] Deng Y, Li G, Song W, et al. Preparation and properties of pearl powder/polypropylene composites and their biocompatibility[J]. Biomed Mater Eng, 2015, 26 Suppl 1:S27-S34.

[15] Silve C, Lopez E, Vidal B, et al. Nacre initiates biomineralization by human osteoblasts maintained in vitro[J].Calcif Tissue Int, 1992, 51(5):363-369.

[16] Yang YL, Chang CH, Huang CC, et al. Osteogenic activity of nanonized pearl powder/poly (lactide-co-glycolide) composite scaffolds for bone tissue engineering[J]. Bio-medical materials and engineering. 2014, 24(1):979-985.

[17] Shen Y, Zhu J, Zhang H, et al. In vitro osteogenetic activity of pearl [J]. Biomaterials, 2006, 27(2):281-287.

[18] Liu YS, Huang QL, Kienzle A, et al. In vitro degradation of porous PLLA/pearl powder composite scaffolds[J]. Mater Sci Eng C Mater Biol Appl,2014, 38:227-234.

[19] Liu Y, Huang Q, Feng Q. 3D scaffold of PLLA/pearl and PLLA/nacre powder for bone regeneration[J]. Biomedl Mater, 2013, 8(6):065001.

[20] Xiao WD, Zhong ZM, Tang YZ, et al. Repair of critical size bone defects with porous poly(D,L-lactide)/nacre nanocomposite hollow scaffold[J]. Saudi Med J,2012,36(6):601-607.

[21] Deng Y, Li G, Song W, et al. Preparation and properties of pearl powder/polypropylene composites and their biocompatibility[J]. Biomed Mater Eng, 2015, 26 Suppl 1: S27-S34.

[22] 李婷, 孙静, 谢富强,等. 骨形成蛋白2/珍珠层粉/壳聚糖支架制备及生物性能研究[J]. 中国生物工程杂志，2015, 35(11):1-6.

[23] Chandler RL, Chandler KJ, McFarland KA. et al. Bmp2 transcription in osteoblast progenitors is regulated by a distant 3' enhancer located 1563 kilobases from the promoter[J]. Mol Cell Biol,2007, 27(8):2934-2951.

[24] Govender S, Csimma C, Genant HK. et al. Recombinant human bone morphogenetic protein-2 for treatment of open tibial fractures: a prospective, controlled, randomized study of four hundred and fifty patients[J]. J Bone Joint Surg Am, 2002, 84-A (12):2123-2134.

[25] Swiontkowski MF, Aro HT, Donell S. et al. Recombinant human bone morphogenetic protein-2 in open tibial fractures. A subgroup analysis of data combined from two prospective randomized studies[J]. J Bone Joint Surg Am, 2006, 88(6):1258-1265.

[26] Park SY, Kim KH, Shin SY. et al. Dual delivery of rhPDGF-BB and bone marrow mesenchymal stromal cells expressing the BMP2 gene enhance bone formation in a critical-sized defect model[J]. Tissue Eng Part A, 2013, 19(21-22):2495-2505.

[27] He X, Dziak R, Yuan X. et al. BMP2 genetically engineered MSCs and EPCs promote vascularized bone regeneration in rat critical-sized calvarial bone defects[J]. PloS one, 2013, 8(4): e60473.

[28] Hwang DY, On SW. Song SI. Bone regenerative effect of recombinant human bone morphogenetic protein-2 after cyst enucleation[J]. Maxillofac Plast Reconstr Surg, 2016, 38(1):22.

[29] Bhakta G, Rai B, Lim ZX. et al. Hyaluronic acid-based hydrogels functionalized with heparin that support controlled release of bioactive BMP-2[J]. Biomaterials, 2012, 33(26):6113-6122.

[30] Brown KV, Li B, Guda T. et al. Improving bone formation in a rat femur segmental defect by controlling bone morphogenetic protein-2 release[J]. Tissue Eng Part A, 2011, 17(13-14):1735-1746.

[31] 李树祎, 周苗等. 三维打印聚乳酸-羟基乙酸/磷酸三钙骨修复支架的生物学评价[J]. 中华口腔医学杂志, 2016, 51(11):661-666.

[32] Zhao X, Komatsu DE, Hadjiargyrou M. Delivery of rhBMP-2 plasmid DNA complexes via a PLLA/collagen electrospun scaffold induces ectopic bone formation[J]. J Biomed Nanotechnol, 2016, 12(6):1285-1296.

[33] 刘启省, 张东刚. 重组人骨形态发生蛋白2/骨修复材料的制备与性能[J]. 中国组织工程研究, 2016, 20(38):5664-5671.

[34] Zhang M, Ma Y, Li R, et al.RhBMP-2-loaded Poly (lactic-co-glycolic acid) microspheres fabricated by coaxial electrospraying for protein delivery[J]. J Biomater Sci Polym Ed, 2017, 28(18):2205-2219.

[35] Tanaka M, Haniu H, Kamanaka T. et al. Physico-chemical, in vitro and in vivo evaluation of a 3D unidirectional porous hydroxyapatite scaffold for bone regeneration[J]. Materials (Basel) ,2017, 10(1).

[36] Wang C, Zhao Q, et al. Cryogenic 3D printing for producing hierarchical porous and rhBMP-2-loaded Ca-P/PLLA nanocomposite scaffolds for bone tissue engineering[J]. Biofabrication, 2017, 9(2):025031.

[37] Chang AR, Cho TH, Hwang SJ. Receptor activator of nuclear factor Kappa-B ligand-induced local osteoporotic canine mandible model for the evaluation of peri-Implant bone regeneration[J]. Tissue Eng Part C Methods, 2017, 23(11):781-794.

[38] Hosseinkhani H, Hosseinkhani M, Khademhosseini A, et al. Bone regeneration through controlled release of bone morphogenetic protein-2 from 3-D tissue engineered nano-scaffold[J]. J Control Release, 2007, 117(3):380-386.

[39] 王建钧, 陈建庭, 杨春露. 珍珠粉水溶性基质对兔骨髓基质干细胞BMP-2和Cbfa1基因表达的影响[J]. 南方医科大学学报, 2007, 27(12):1838-1840.

[40] Green DW, Kwon HJ， Jung HS. Osteogenic potency of nacre on human mesenchymal stem cells[J]. Mol Cells, 2015, 38(3):267-272.

[41] Zhao M, Shi Y, He M， et al. PfSMAD4 plays a role in biomineralization and can transduce bone morphogenetic protein-2 signals in the pearl oyster Pinctada fucata[J]. BMC Dev Biol, 2016, 16:9.

选择文件类型/文献管理软件名称

选择包含的内容