BDNF/TrkB/Akt/p-RPS6信号通路在口腔上皮牙源性病变患儿中的作用及免疫反应分析

doi:10.3969/j.issn.1005-4979.2023.01.003

摘要/Abstract

摘要： 目的: 分析BDNF/TrkB/Akt/p-RPS6信号通路在儿童口腔上皮牙源性病变中的作用及免疫反应。方法: 以2018年6月—2020年6月间本院收治的50例口腔上皮牙源性病变患儿的口腔上皮病变组织标本（病例组）和50例健康儿童的口腔上皮组织标本（对照组）为研究对象，均行BDNF/TrkB/Akt/p-RPS6信号通路蛋白检测、免疫功能T细胞亚群（CD3⁺、CD4⁺、CD4⁺/CD8⁺）检测和免疫球蛋白指标（IgG、IgA、IgM）检测，对比对照组和病例组上述指标的差异，并采用Pearson相关性分析法分析BDNF/TrkB/Akt/p-RPS6信号通路蛋白与免疫功能相关指标的相关性。结果: 病例组的脑源性神经营养因子（brain-derived neurotrophic factor，BDNF）、酪氨酸激酶受体B（tyrosine kinase receptor B，TrkB）、蛋白激酶B（protein kinase B，PkB，又称Akt）、磷酸化S6核糖体蛋白抗体（anti-phospho-S6 ribosoma protein，p-RPS6）的平均光密度值均高于对照组（P<0.05）。病例组的免疫功能T亚群细胞（CD3⁺、CD4⁺、CD4⁺/CD8⁺）、免疫球蛋白指标（IgG、IgA、IgM）数据值均低于对照组（P<0.05）。BDNF、TrkB、Akt、P-RPS6的平均光密度值与免疫功能T细胞亚群（CD3⁺、CD4⁺、CD4⁺/CD8⁺）数据值、免疫球蛋白指标（IgG、IgA、IgM）数据值呈负相关（均P<0.05）。结论: 口腔上皮牙源性病变患儿存在明显的BDNF/TrkB/Akt/p-RPS6信号通路相关蛋白表达异常和免疫功能抑制，且免疫功能抑制与BDNF/TrkB/Akt/p-RPS6信号通路蛋白异常表达相关。

关键词: BDNF/TrkB/Akt/p-RPS6信号通路, 口腔上皮牙源性病变

Abstract:

Objective: To analyze the role and immune response of BDNF/TrkB/Akt/p-RPS6 signaling pathways in children′s oral epithelial odontogenic lesions. Methods: Tissue specimens from 50 children with oral epithelial odontogenic lesions (odontogenic lesion group) and 50 oral epithelial tissue samples (control group) who were treated in our hospital from June 2018 to June 2020 were selected. In all samples, proteins in BDNF/TrkB/Akt/p-RPS6 signaling pathway, immune function T cell subsets(CD3⁺, CD4⁺, CD4⁺/CD8⁺) and immunoglobulin indexes (IgG, IgA, IgM) were detected to compare the differences of the above indexes between healthy group and odontogenic lesion group. Pearson correlation analysis was used to analyze the correlation between signal pathway protein and immune function related indexes. Results: The average optical density of brain-derived neurotrophic factor (BDNF), tyrosine kinase receptor (TrkB)，protein kinase B (PkB, also know as Akt) and anti-phospho-S6 ribosoma protein(p-RPS6) in odontogenic lesion group was higher than that in control group (P<0.05). The data of immune function T cell subsets(CD3⁺, CD4⁺, CD4⁺/CD8⁺) in odontogenic lesion group were lower than those in control group(P<0.05). The data of immunoglobulin indexes (IgG, IgA, IgM) in odontogenic lesion group were lower than those in control group(P<0.05). The mean optical density value of BDNF, TrkB, Akt and p-RPS6 was negatively associated with the data of immune functional T cell subsets(CD3, CD4, CD4/CD8) and immunoglobulin indexes(IgG, IgA, IgM)(all P<0.05). Conclusion: There were obviously abnormal expression of BDNF/TrkB/Akt/p-RPS6 signal pathway related proteins and immunosuppression in patients with oral epithelial odontogenic lesions, and immunosuppression was related to abnormal expression of BDNF/TrkB/Akt/p-RPS6 signal pathway proteins.

Key words: BDNF/TrkB/Akt/p-RPS6 signaling pathway, oral epithelial dental lesions

中图分类号:

R782

伍宗辉, 汪瑞芳. BDNF/TrkB/Akt/p-RPS6信号通路在口腔上皮牙源性病变患儿中的作用及免疫反应分析[J]. 《口腔颌面外科杂志》, 2023, 33(1): 14-19.

WU Zonghui, WANG Ruifang. Analysis of BDNF/TrkB/Akt/p-RPS6 signaling pathway in children with oral epithelial odontogenic lesion[J]. 《Journal of Oral and Maxillofacial Surgery》, 2023, 33(1): 14-19.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2023/V33/I1/14

图/表 7

图1 下颌骨牙源性角化囊性瘤的影像和病理特征 A—C. 分别为术前、开窗引流术后3个月及肿物切除术后6个月的曲面断层片图像，箭头所指为牙源性角化囊性瘤；D. 术后HE染色图（×400）。

Figure 1 Panoramic radiography images and pathologic features showing a case of keratocystic odontogenic tumor in mandible

表1 对照组和病例组BDNF/TrkB/Aktt/p-RPS6信号通路蛋白平均光密度值的比较（$\bar{x}±s$）

Table 1 Comparison of average optical density of BDNF/TrkB/Aktt/p-RPS6 signaling pathway proteins between control group and odontogenic lesion group （$\bar{x}±s$）

组别	BDNF	TrkB	Akt	p-RPS6	内参GAPDH
对照组（n=50）	0.63±0.14	0.84±0.33	0.97±0.28	0.68±0.24	0.99±0.36
病例组（n=50）	1.19±0.28^①	1.87±0.56^①	2.34±0.59^①	1.58±0.49^①	2.27±0.76
t值	12.649	11.205	14.834	11.668	10.763
P值	0.000	0.000	0.000	0.000	0.000

图2 BDNF、TrkB、Akt、p-RPS6的蛋白免疫组织化学图（×200） A、B. 2组BDNF蛋白的免疫组织化学图；C、D. 2组TrkB蛋白的免疫组织化学图；E、F. 2组Akt蛋白的免疫组织化学图；G、H. 2组p-RPS6蛋白的免疫组织化学图。

Figure 2 Immunohistochemistry staining of BDNF、TrkB、Akt、p-RPS6 protein （×200）

图3 BDNF/TrkB/Aktt/p-RPS6信号通路蛋白条带图

Figure 3 BDNF/TrkB/Aktt/p-RPS6 signaling pathway protein bands

表2 对照组和病例组T细胞亚群指标比较（$\bar{x}±s$）

Table 2 Comparison of T cell subsets between control group and odontogenic lesion group ($\bar{x}±s$)

组别	CD3⁺/%	CD4⁺/%	CD4⁺/CD8⁺
对照组（n=50）	63.29±2.03	55.18±1.83	1.14±0.16
病例组（n=50）	58.52±1.93^①	46.38±1.52^①	0.98±0.14^①
t值	14.627	13.038	6.207
P值	0.000	0.000	0.000

表3 对照组和病例组免疫球蛋白指标比较（U/L，$\bar{x}±s$）

Table 3 Comparison of immunoglobulin indexes between control group and odontogenic lesion group (U/L，$\bar{x}±s$)

组别	IgG	IgA	IgM
对照组（n=50）	12.59±1.86	2.08±0.44	1.20±0.32
病例组（n=50）	8.52±1.93^①	1.26±0.34^①	0.88±0.20^①
t值	10.709	8.271	9.384
P值	0.000	0.000	0.000

表4 BDNF/TrkB/Akt/p-RPS6信号通路蛋白与免疫功能相关指标的Pearson相关系数

Table 4 Pearson correlation coefficient between BDNF/TkB/Akt/p-RPS6 signaling pathway proteins and immune function related indicators

	BDNF	TrkB	Akt	p-RPS6	CD3⁺	CD4⁺	CD4⁺/CD8⁺	IgG	IgA
BDNF
TrkB	0.082
Akt	0.034	0.041
p-RPS6	0.050	0.052	0.014
CD3⁺	-0.751	-0.681	-0.678	-0.692
CD4⁺	-0.802	-0.715	-0.632	-0.675	0.017
CD4⁺/CD8⁺	-0.645	-0.638	-0.692	-0.758	0.034	0.197
IgG	-0.727	-0.684	-0.704	-0.608	0.058	0.208	0.092
IgA	-0.708	-0.784	-0.719	-0.816	0.143	0.241	0.063	0.118
IgM	-0.734	-0.685	-0.689	-0.772	0.128	0.284	0.107	0.099	0.083

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