磨牙咬合升高对小鼠短期内焦虑情绪的影响

doi:10.3969/j.issn.1005-4979.2015.06.002

《口腔颌面外科杂志》 ›› 2015, Vol. 25 ›› Issue (6): 400-. doi: 10.3969/j.issn.1005-4979.2015.06.002

磨牙咬合升高对小鼠短期内焦虑情绪的影响

尚琳1，苏俭生1，韩雯斐1，孟博2

1. 同济大学口腔医学院·口腔医院修复教研室，上海牙组织修复与再生工程技术研究中心，上海 200072；
2. 华东师范大学脑功能基因组学教育部重点实验室，上海市脑功能基因组学重点实验室，上海 200062

收稿日期:2015-05-01 修回日期:2015-08-01 出版日期:2015-12-28 发布日期:2016-03-11
通讯作者: 苏俭生，教授. E-mail:sjs@tongji.edu.cn
作者简介:尚琳（1989—），女，山东人，硕士.
基金资助:
上海市科委重点项目（13411951201）

Occlusion Vertical Rising of Molars Increased Anxiety Temporally in Mice

SHANG Lin1, SU Jian-sheng1, HAN Wen-fei1, MENG Bo2

1. Department of Prosthodontics, School and Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072; 2. Shanghai Key Laboratory of Brain Functional Genomics, Key Laboratory of Brain Functional Genomics, Ministry of Education, East China Normal University, Shanghai 200062, China

Received:2015-05-01 Revised:2015-08-01 Online:2015-12-28 Published:2016-03-11

摘要/Abstract

摘要： 目的：建立磨牙咬合升高小鼠模型，观察其短期内焦虑样情绪及臂旁核、杏仁核中神经元激活水平的变化。方法：8周龄C57BL/6J雄性小鼠，随机分为2组：假手术组和咬合升高组。造模后连续记录体重及进食量；于第1天、第3天、第7天及第14天，行高架十字迷宫测试、旷场测试、新环境抑制进食测试、社交探索测试等焦虑样情绪相关的行为学检测；免疫荧光染色观察小鼠杏仁核中央区Fos蛋白表达水平。结果：磨牙区咬合升高对小鼠体重、进食量及基础活动能力并无短期影响。在处理后7、14 d，咬合升高组小鼠焦虑样行为明显增加。与假手术组相比，咬合升高导致小鼠臂旁核、杏仁核神经元激活水平升高。结论：磨牙区咬合升高会导致小鼠短期内焦虑样情绪水平增加。

关键词: 咬合升高； , 焦虑； , 行为

Abstract: Objective: To explore the onset and development of affective disorders concomitant with occlusion vertical rising (OVR) in mice models. Methods: 8-week-old C57BL/6J male mice were randomly assigned into two groups: OVR group and sham group. On postoperative Day1/Day3/Day7/Day14, the anxiogenic phenotypes were verified via animal behavior during the elevated plus maze test, and by additional analysis of the open field test, the novelty-suppressed feeding test and the social exploration test. The expression of Fos in central amygdaloid nucleus (CeA) and parabrachial nucleus (PBN) were detected by immunofluorescence. Results: Over perioperative period, the weight, food intake and locomotor activity in two groups did not show significant difference. Occlusion vertical rising group exhibited more anxiety-like behaviors compared to sham group in multiple tests. Meanwhile, the level of Fos-positive cells in CeA and PBN in occlusion vertical rising group were higher than that in sham group. Conclusion: The results indicate that occlusion vertical rising may enhance to more anxiety-related behaviors in mice temporally.

Key words: occlusion vertical rising, anxiety, behavior

中图分类号:

R782

尚琳1，苏俭生1，韩雯斐1，孟博2. 磨牙咬合升高对小鼠短期内焦虑情绪的影响[J]. 《口腔颌面外科杂志》, 2015, 25(6): 400-.

SHANG Lin1, SU Jian-sheng1, HAN Wen-fei1, MENG Bo2. Occlusion Vertical Rising of Molars Increased Anxiety Temporally in Mice[J]. 《Journal of Oral and Maxillofacial Surgery》, 2015, 25(6): 400-.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2015/V25/I6/400

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