摘要： 目的：研究Sox9基因、基质金属蛋白酶基因12（MMP12）在兔盘前移颞下颌关节的髁突软骨细胞中时空表达变化。方法：60只日本大耳白兔随机分为5组（对照组，1、2、4、8周组；每组12只），分别在颞下颌关节盘前移位建模后的1、2、4、8周处死，取出髁突软骨并将关节部位制成组织芯片，利用DNA微阵列技术、实时荧光定量PCR、免疫组化技术和IPP图像处理技术观察不同组内髁突软骨中Sox9、MMP12表达。结果：基因检测和rtPCR验证结果基本一致，显示为MMP12的表达在第1周达到最高值，之后随建模时间递减，在第8周达到最小值（表达下降），Sox9的表达第1周较低，随后略有上升，在第2、4、8周分别检测到其表达上升并存在统计学差异。结论：MMP12和Sox9 同为骨性表达相关因子，在盘前移位颞下颌关节中，MMP12主要参与早期骨溶解破坏、炎症反应，Sox9主要参与后期软骨的修复重建。

关键词:  , 颞下颌关节,  ,  , Sox9,  ,  , MMP12,  ,  , 免疫组化技术,  ,  , DNA微阵列

Abstract: Objective: To investigate the dynamic expression of Sox9 and MMP12 in rabbits' condylar chondrocyte of temporomandibular joint internal derangement ( TMJID). Methods: 60 Japanese white rabbits were randomly divided into five groups (control group, 1-week group, 2-week group, 4-week group, and 8-week group; n=12), which were sacrificed respectively after modeling TMJID at one week, 2 weeks, 4 weeks and 8 weeks respectively, then take out of condylar and made tissue chips from joints. DNA microarray technology, quantitative rtPCR, immunohistochemical technique and image processing were used to observe the expression of Sox9 and MMP12 in condylar chondrocyte among different groups. Results: The results of genetic testing and rtPCR were basically consistent, which showed that MMP12 expression reached the highest level in the first week, then decreased with the modeling time going and reached the lowest level in the eighth week (decreased expression). Sox9 expression was low in the first week, then rose slightly, especially it rose significantly in the second, fourth, and eighth week. Conclusion: In TMJID, MMP12 is mainly involved in the early osteolysis destruction and Sox9 mainly involved in the late cartilage repair and reconstruction.

Key words: temporomandibular joint, Sox9, MMP12, immunohistochemical technique, DNA microarray technology

中图分类号: 

• R782.6