氧化石墨烯对牙周致病菌影响的实验研究

《口腔颌面外科杂志》

氧化石墨烯对牙周致病菌影响的实验研究

吴志富，王佐林

上海牙组织修复与再生工程技术研究中心，同济大学附属口腔医院，上海200072

出版日期:2019-02-01 发布日期:2019-11-28
通讯作者: 王佐林，教授.E-mail：zuolin@tongji.edu.cn
作者简介:吴志富（1991— ），男，安徽安庆人，硕士研究生.Email：2291921189@qq.com
基金资助:
中央高校基本科研业务费专项资金（1504219037）

The Influence of Graphene Oxide Lateral Size and Solution Concentration on Its Antibacterial Effect

WU Zhi-fu，WANG Zuo-lin

Hospital of Stomatology, Tongji University, Shanghai Engineering Research Center of Tooth Restoration and Regeneration, Shanghai 200072, China

Online:2019-02-01 Published:2019-11-28

摘要/Abstract

摘要： 目的：本课题通过制备氧化石墨烯GO（Graphene oxide）并将其与伴放线聚集杆菌细菌（actinobacillus actinomycetemcomitans,Aa）共培养,观察细菌的生长与增殖等情况，了解不同浓度及横向尺寸对GO抗菌效果的影响。方法：采用改良Hummers法将石墨制备成GO，配置溶液进行超声震荡,通过原子力显微镜、拉曼光谱等技术表征GO。将Aa分别与不同浓度和不同横向尺寸的GO溶液进行共培养2h。通过MTT实验，菌落计数，活死细菌染色等方法，对GO的抗菌效果进行评估。结果：横向尺寸相同时，高浓度的GO溶液处理组的细菌失活率，明显高于低浓度处理组，差异具有统计学意义；相同浓度，不同横向尺寸的GO溶液处理细菌2h后，细菌的失活率无明显差异。结论：GO的抗菌作用呈现浓度和处理时间依赖性，GO横向尺寸对其抗菌效果影响较小。

关键词: 种植体周围炎, GO, 超声震荡, 抗菌, 横向尺寸

Abstract: Objective: This study intends to prepare GO particles and co-culture GO solution with bacteria Aa. The antibacterial effect of GO solution was evaluated. Methods: Graphite was prepared into GO by the modified Hummers, and a stock solution was prepared and ultrasonically shaken. The characteristics of GO sheets were obtained through the atomic force microscope, raman spectroscopy and other relative techniques. The suspension of bacteria Aa was co-cultured with GO-0 solution with different concentration (0, 15, 30, 60, 120 ug/ml), and different lateral size (0.72、0.47、0.15、0.04，0.01um2). the antibacterial effect of GO was evaluated through MTT reduction experiments, colony count units and live/dead bacterial staining. Results: When the transverse dimensions were the same, the bacterial inactivation rate of the high concentration GO solution treatment group was significantly higher than that of the low concentration treatment group, and the difference was statistically significant. After the same concentration and different lateral size GO solution treatment of the bacteria for 2 hours, the bacteria were inactivated. There is no significant difference in the rate. Conclusion: The antibacterial effect of GO is concentration and treatment time dependent, and the transverse dimension of GO has little effect on its antibacterial effect.

Key words: implantitis, graphene oxide, ultrasonic vibration, antibacterial, lateral size

吴志富，王佐林. 氧化石墨烯对牙周致病菌影响的实验研究[J]. 《口腔颌面外科杂志》.

WU Zhi-fu，WANG Zuo-lin. The Influence of Graphene Oxide Lateral Size and Solution Concentration on Its Antibacterial Effect[J]. 《Journal of Oral and Maxillofacial Surgery》.

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