《口腔颌面外科杂志》 ›› 2012, Vol. 22 ›› Issue (5): 354-354. doi: 10.3969/j.issn.1005-4979.2012.05.011

• 口腔种植学研究 • 上一篇    下一篇

种植体周龈沟液高迁移率族蛋白B1的检测分析

刘金盼1 , 王明国2 , 李静1 , 杨世茂3   

  1. 1.潍坊医学院口腔医学院,山东 潍坊 261053;2.济南市中心医院口腔科,山东 济南 250013;3.山东大学口腔医学院,山东 济南 250012
  • 出版日期:2012-10-28 发布日期:2013-01-02
  • 通讯作者: 王明国,副教授. E-mail:wmgsh@163.com
  • 作者简介:刘金盼(1983—),男,山东聊城人,硕士研究生. E-mail:kingpanlau@gmail.com

Expression of High Mobility Group Box 1 Levels in Crevicular Fluid of Peri-implantitis Tissues

LIU Jin-pan1, WANG Ming-guo2, LI Jing1, YANG Shi-mao3   

  1. 1.School of Stomatology, Weifang Medical College, Weifang 261053; 2. Department of Stomatology, Jinan Municipal Central Hospital, Jinan 250013; 3.College of Stomatology, Shandong University, Jinan 250012, Shandong Province, China
  • Online:2012-10-28 Published:2013-01-02

摘要: 目的:检测种植体周围炎患者龈沟液(gingival crevicular fluid, GCF)中高迁移率族蛋白B-1 (high mobility group box l, HMGB-1)的水平,以探讨HMGB-1水平与种植体周围炎间的关系。方法:经患者知情同意,选取行种植手术1年以上的健康种植体39颗和种植体周围炎40颗,共79颗种植体,收集种植体周围GCF,并运用ELISA法检测其中HMGB-1、IL-1β、IL-8、TNF-α的浓度,同时检测种植体周围的临床指标。结果:轻度种植体周围炎组的HMGB-1、IL-1β、IL-8及TNF-α浓度高于健康种植体组,重度种植体周围炎的浓度水平又高于轻度种植体周围炎组,HMGB-1与IL-1β、TNF-α的浓度之间亦存在正相关关系(P<0.01)。结论:种植体周围GCF中HMGB-1水平变化与种植体周围炎的病变程度有关,且与经典炎症因子间存在显著相关性,临床检测GCF中HMGB-1水平可作为诊断种植体周围炎的客观指标。

关键词: 高迁移率族蛋白B-1, 炎症因子, 相关性, 种植体周围炎

Abstract: Objective:To analyze whether the expression of high mobility group box 1 protein (HMGB-1) in gingival crevicular fluid (GCF) are associated with the severity of peri-implantitis.   Methods:GCF was sampled from 79 implantation patients including 39 normal peri-implant tissue and 40 peri-implantitis tissues.  HMGB-1,IL-1β,IL-8,and TNF-α levels were analyzed using ABC-ELISA. Clinical parameters were measured to investigate their relationships. Statistical analyses were performed with SPSS 13.0 software. Results:Significant difference of HMGB-1 expression was observed between healthy implant sites and peri-implantitis sites (P<0.01). The expression levels of HMGB-1 showed positive correlation  with those of IL-1β,IL-8, and TNF-α. Conclusion: HMGB-1 cytokine in GCF may be of value as a parameter in diagnosis and prognosis for peri-implantitis.

Key words: correlation coefficient, cytokine, HMGB-1, peri-implantitis

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