Abstract:

Objective: To investigate the expression levels of lumican (LUM) and fibromodulin (FMOD) in oral squamous cell carcinoma (OSCC) tissues, and their correlation with the prognosis of OSCC patients.Methods: A total of 30 fresh OSCC tissues and 30 normal tissues were detected by Western blotting and real-time quantitative polymerase chain reaction (RT-qPCR). The 50 paraffin-embedded samples of OSCC and 9 paraffin-embedded samples of normal oral mucosa were detected by immunohistochemistry (IHC). The correlation of LUM and FMOD expressions in OSCC tissues was analyzed by Spearman correlation analysis. Kaplan-Meier method was used for survival analysis. Cox regression model was used for multivariate analysis.Results: The protein and mRNA expression levels of LUM and FMOD in the OSCC tissue were significantly higher than those in the normal tissues (P<0.001). However, there was no difference in the protein and mRNA levels of both LUM and FMOD in OSCC tissues of different degrees of differentiation (P>0.05). IHC results suggested significantly increased expression of LUM and FMOD in OSCC tissues, which were correlated with both tumor clinical staging (P<0.001) and lymph node metastasis (P<0.05). Spearman correlation analysis showed a significant correlation between the expression of LUM and FMOD in OSCC tissues. Kaplan-Meier analysis showed that overall survival rate (OS) and tumor-specific survival rate (TSS) of patients with high LUM and FMOD expression levels were lower than those of patients with low expression levels. The Cox regression model showed that the expression of LUM and FMOD and clinical staging can be used as independent factors for predicting the prognosis of OSCC patients.Conclusion: The expressions of LUM and FMOD in OSCC are significantly increased, which may play a promoting role in the development of OSCC and suggest the prognosis of patients with OSCC.

Key words: lumican, fibromodulin, oral squamous cell carcinoma, extracellular matrix

摘要：

目的： 研究光蛋白聚糖（lumican，LUM）和纤维调节蛋白（fibromodulin，FMOD）在口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）组织中的表达及其对患者预后的影响。方法： 应用蛋白质印迹法及实时定量聚合酶链反应（real-time quantitative polymerase chain reaction，RT-qPCR）对30例新鲜的OSCC组织及30例正常组织进行检测，应用免疫组织化学（immunohistochemistry，IHC）法对50例OSCC石蜡标本及9例正常组织石蜡标本进行检测，应用Spearman相关性分析法分析LUM和FMOD在OSCC组织中表达的相关性，应用Kaplan-Meier法进行生存率分析，采用Cox回归模型进行多因素分析。结果： OSCC组织中，LUM和FMOD的蛋白表达水平和mRNA表达水平明显高于正常组织（均P<0.001），但是二者的蛋白、mRNA表达水平均在不同分化程度的OSCC组织中无差异（P>0.05）。IHC结果提示，LUM和FMOD在OSCC组织中的表达水平明显升高，且均与肿瘤临床分期（均P<0.001）和淋巴结转移（均P<0.05）相关。Spearman相关性分析结果显示，LUM和FMOD在OSCC组织中的表达具有相关性。Kaplan-Meier分析结果表明，LUM和FMOD高表达组患者的总生存率（overall survival rate，OS）和肿瘤特异性生存率（tumor-specific survival rate，TSS）低于低表达组患者。Cox回归模型显示，LUM和FMOD的表达水平及临床分期是影响OSCC患者预后的独立因素。结论： LUM和FMOD在OSCC中的表达显著升高，提示其可能在OSCC发生、发展中起促进作用，并可提示OSCC患者的预后情况。

关键词: 光蛋白聚糖, 纤维调节蛋白, 口腔鳞状细胞癌, 细胞外基质

CLC Number: 

• R782

• R739.8