Identification of ZO-1 in Oral Verrucous Carcinoma and Oral Squamous Cell Carcinoma

doi:10.3969/j.issn.1005-4979.2012.06.003

Abstract

Abstract: Objective: This study was purposed to analysis the transcriptional expression of Zonula Occluden -1 (ZO-1) in oral verrucous carcinoma（OVC） and oral squamous cell carcinoma（OSCC）. Methods: Pathological tissue samples were obtained from patients of OSCC（n=20）, OVC（n=10）, as well as matched OSCC pericarcinomatous tissue（n=20） and normal oral mucosa （n=10）. The transcriptional expression of ZO-1 was detected by Real-time PCR method. Results: The transcriptional expressions of ZO-1 in OSCC and OVC were lower than their matched pericarcinomatous tissue and normal oral mucosa tissue (P<0.001). The ZO-1 transcriptional level in OSCC was significantly lower than that in OVC (P<0.05). Conclusion: There might be different epithelial-mesenchymal transition process among OVC and OSCC.

Key words: ZO-1, oral verrucous carcinoma, oral squamous cell carcinoma, epithelial-mesenchymal transition

摘要： 目的：研究闭锁小带蛋白-1（zonula occluden -1，ZO-1）在口腔疣状癌和口腔鳞癌中的表达，分析ZO-1相关的上皮间充质转化过程参与口腔癌发生发展的情况。方法：取20例口腔鳞癌及其癌旁组织，10例口腔疣状癌组织及10例正常口腔黏膜组织标本，荧光实时定量Real-time PCR方法检测ZO-1基因mRNA在不同组织中的表达。结果：口腔疣状癌与鳞癌组织中ZO-1基因mRNA的表达低于正常口腔黏膜上皮组织与癌旁组织(P<0.05)；口腔疣状癌中ZO-1基因mRNA的表达高于鳞癌(P<0.05)。结论：ZO-1作为上皮间充质转化过程中的关键蛋白，其在口腔疣状癌与鳞癌中表达水平的差异,表明口腔鳞癌与口腔疣状癌中存在不同程度的上皮间充质转化现象。

关键词: 闭锁小带蛋白（ZO-1）, 口腔疣状癌, 口腔鳞癌, 上皮间充质转化

CLC Number:

R739.85

WANG Mu, TANG Zhan-gui， LIU Ou-sheng， DENG Zhi-yuan. Identification of ZO-1 in Oral Verrucous Carcinoma and Oral Squamous Cell Carcinoma[J]. 《Journal of Oral and Maxillofacial Surgery》, 2012, 22(6): 390-392.

王　木，唐瞻贵，刘欧胜，邓智元. 闭锁小带蛋白-1基因在口腔疣状癌和口腔鳞癌中的转录水平分析[J]. 《口腔颌面外科杂志》, 2012, 22(6): 390-392.

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URL: https://journal06.magtech.org.cn/Jweb_joms/EN/10.3969/j.issn.1005-4979.2012.06.003

https://journal06.magtech.org.cn/Jweb_joms/EN/Y2012/V22/I6/390

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