Abstract: Objective: The purpose of this study was to examine the complexation effects of epigallocatechin-3-gallate (EGCG) with Cr ³⁺ on the expression of 67LR and EGFR on human gingival fibroblast cells. Methods: Real-time PCR was adopted to study the expression of 67LR， EGFR at mRNA levels and Western blot on protein levels. Results: EGCG suppressed the expression of 67LR， EGFR mRNA and proteins. The co-treatment of Cr ³⁺ and EGCG decreased EGCG-induced inhibition effect on 67LR expression. Cr ³⁺ in low concentration (lesser than 200 μmol/L) could decrease EGCG-induced inhibition effect on EGFR expression. Conclusion： EGCG inhibits the expression of 67LR and EGFR. EGCG mixed with Cr ³⁺ could reduce EGFR eexpression and has no significant effects on 67LR expression.

Key words: Cr³⁺, EGCG, 67LR, EGFR

摘要： 目的： 分析表没食子儿茶素没食子酸酯（EGCG）与Cr³⁺共同作用，对牙龈成纤维细胞(HGF)的EGCG受体67LR和表皮生长因子受体（EGFR）的影响。方法：EGCG与Cr³⁺共同作用牙龈成纤维细胞24 h后，用Real-time PCR法检测mRNA表达，运用Western印迹法分析蛋白表达。结果：EGCG能抑制HGF细胞表面受体67LR mRNA以及蛋白水平的表达，加入Cr³⁺后减弱了EGCG对细胞67LR mRNA和蛋白的影响。EGCG作用24h能降低EGFR mRNA和蛋白的表达，Cr³⁺(<200 μmol/L)会减弱EGCG抑制EGFR的作用。结论：EGCG能降低HGF细胞67LR和EGFR的表达。加入 Cr³⁺后对细胞67LR无明显影响，但降低细胞EGFR的表达。

关键词: Cr³⁺, 表没食子儿茶素没食子酸酯（EGCG）, 层粘连蛋白受体（67LR）, 表皮生长因子受体（EGFR）

CLC Number: 

• R739.8