Osteogenic Differentiation of Rat ADSCs Induced by Echinacoside

doi:10.3969/j.issn.1005-4979.2019.04.001

Abstract

Abstract: Objective: To study the effect of echinacoside on the osteogenic differentiation of rat adipose derived stem cells (ADSCs) and its further underlying mechanism. Methods: ADSCs were subjected to 0.01，0.1 and 1 mg/mL echinacoside culture solution，the control group was ADSCs cultured without echinacoside. The proliferation of ADSCs was tested with MTT assay, the osteogenic differentiation was investigated using alkaline phosphatase (ALP) staining, semi-quantitation of ALP, alizarin red S (ARS) staining, and semi-quantitation of ARS. The expression of phosphorylated extracellular signal-regulated kinase (ERK), p38 kinase and c-Jun N terminal kinase (JNK) was analyzed with Western blot. Results: Results showed that the proliferation of ADSCs was not impacted by echinacoside (P>0.05). ALP staining and ARS staining were stronger in 0.01, 0.1 and 1 mg/mL echinacoside culture solution groups. ALP and ARS activity quantitative assay demonstrated significantly higher in echinacoside groups（P<0.05）. Western blot results demonstrated that echinacoside rapidly phosphorylated ERK, p38 and JNK. Conclusion: Echinacoside could promote the osteogenic differentiation of ADSCs, while not impact its proliferation, a potential mechanism involving ERK, p38 and JNK MAPK pathways.

Key words: echinacoside, osteogenic differentiation, MAPK pathways, adipose derived stem cells

摘要： 目的：探讨松果菊苷对大鼠脂肪来源干细胞（ADSCs）成骨分化的作用及其可能机制。方法：以0.01、0.1和1 mg/mL松果菊苷培养液培养ADSCs，未加松果菊苷的培养液作为对照组，应用 MTT 法检测松果菊苷对细胞增殖活性的影响，碱性磷酸酶（ALP）染色和半定量检测细胞 ALP 的表达，茜素红染色（ARS）和半定量检测细胞的基质矿化，Western blot 检测ERK、p38 和 JNK 的蛋白磷酸化水平。结果：1、4、7 d，0.01及0.1和1 mg/mL松果菊苷培养液对大鼠ADSCs的增殖活性无显著影响（P>0.05）； 0.01、0.1和1 mg/mL松果菊苷培养液组ALP染色和ARS染色较对照组深染，ALP蛋白分泌和ARS基质矿化均较对照组有显著的增加（P<0.05）；0.01 mg/mL松果菊苷培养液作用下ERK、p38和JNK的蛋白磷酸化水平升高。结论：松果菊苷对ADSCs增殖无影响，可促进ADSCs向成骨方向分化，其作用机制与MAPK 信号通路有关。

关键词: 松果菊苷;成骨分化, MAPK信号通路, 脂肪干细胞

CLC Number:

R782

Lv kai ge,Wang gui fang,Hao yi. Osteogenic Differentiation of Rat ADSCs Induced by Echinacoside[J]. 《Journal of Oral and Maxillofacial Surgery》, doi: 10.3969/j.issn.1005-4979.2019.04.001.

吕凯歌，王桂芳，郝轶. 松果菊苷促进大鼠脂肪干细胞成骨分化的研究[J]. 《口腔颌面外科杂志》, doi: 10.3969/j.issn.1005-4979.2019.04.001.

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URL: https://journal06.magtech.org.cn/Jweb_joms/EN/10.3969/j.issn.1005-4979.2019.04.001

https://journal06.magtech.org.cn/Jweb_joms/EN/Y2019/V29/I4/181

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