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	ALG2在口腔鳞状细胞癌中的表达及其预后诊断价值
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doi:10.12439/kqhm.1005-4979.2024.05.005

摘要/Abstract

摘要：

目的：分析凋亡关联基因 2（apoptosis-linked gene-2，ALG2）在口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）组织中的表达及其与临床病理特征的相关性、预后诊断价值。方法：实时定量聚合酶链反应（real-time quantitative polymerase chain reaction，RT-qPCR) 检测 ALG2 在 5 对新鲜 OSCC 组织、癌旁组织及 OSCC 细胞系、人口腔黏膜角质形成细胞（human oral keratinocytes，HOK）中的表达；免疫组织化学（immunohistochemistry，IHC）法检测ALG2 在 128 对 OSCC 组织和癌旁组织中的表达，并分析其表达高低与临床病理特征及预后的相关性；利用 TCGA 数据库分析 ALG2 在 OSCC 组织中的表达及其预后诊断价值。结果：ALG2 在 OSCC 组织和细胞系中的表达明显高于其在癌旁组织和 HOK 细胞中（P<0.05）；ALG2 低表达组 OSCC 患者的总体生存率、无疾病生存率和无进展生存率均显著高于 ALG2 高表达组（P<0.05）；T 分期、淋巴结转移、ALG2 表达水平均是影响 OSCC 患者预后的因素（P<0.05），且ALG2 表达是 OSCC 患者预后的一个独立预测因素（P<0.01）；ALG2 对 OSCC 可能具有一定的诊断价值 [ 曲线下面积（area under the curve，AUC）=0.807]。结论：ALG2 在 OSCC 中高表达，且与患者预后不良有关，对 OSCC 可能具有一定的诊断价值。

关键词: 口腔鳞状细胞癌, 凋亡关联基因 2, 预后

Abstract: Objective: To analyze the expression of apoptosis-linked gene-2 (ALG2) in oral squamous cell carcinoma (OSCC) tissues and cell lines and its correlations with the clinicopathological features and prognosis of patients. Methods: The expression of ALG2 in 5 pairs of fresh OSCC tissues, paracancerous normal tissues, OSCC cell lines and human oral keratinocytes (HOK) were detected by real-time quantitative polymerase chain reaction (RT-qPCR). The expression levels of ALG2 in paraffin-embedded OSCC tissues and adjacent normal tissues specimens of 128 patients with OSCC were detected by immunohistochemistry (IHC). The correlation of ALG2 expression in OSCC tissues with the clinicopathological features and survival time of patients were analyzed. The expression of ALG2 in OSCC tissues and its diagnostic, prognostic value were detected in TCGA database. Results: The expression of ALG2 in OSCC tissues and cell lines was significantly higher than those in paracancerous tissues and HOK cells (P<0.05). The overall survival, disease free survival and progression-free survival of OSCC patients in the group with low ALG2 expression were significantly higher than those in the ALG2 high expression group (P<0.05). T stage, lymphatic metastasis, and ALG2 expression were all factors affecting the prognosis of OSCC patients (P<0.05). ALG2 expression was an independent predictor of the prognosis of OSCC patients (P<0.01). ALG2 may have some diagnostic value for OSCC [area under the curve (AUC)=0.807]. Conclusion: ALG2 is highly expressed in OSCC and it is associated with unfavorable prognosis of the patients, and it may have some diagnostic value for OSCC.

Key words: oral squamous cell carcinoma, apoptosis-linked gene-2, prognosis

中图分类号:

R739.8
R782

高瑞, 郭磊, 彭博, 贾骏.

ALG2在口腔鳞状细胞癌中的表达及其预后诊断价值

[J]. 《口腔颌面外科杂志》, 2024, 34(5): 357-364.

GAO Rui, GUO Lei, PENG Bo, JIA Jun.

Expression of ALG2 in oral squamous cell carcinoma and its prognostic diagnostic value

[J]. 《Journal of Oral and Maxillofacial Surgery》, 2024, 34(5): 357-364.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2024/V34/I5/357

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ALG2在口腔鳞状细胞癌中的表达及其预后诊断价值

Expression of ALG2 in oral squamous cell carcinoma and its prognostic diagnostic value

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ALG2在口腔鳞状细胞癌中的表达及其预后诊断价值

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