摘要：

目的： 探究紫杉醇联合ipatasertib对口腔鳞状细胞癌（oral squamous cell carcinoma，OSCC）细胞Cal-27致死率、增殖水平及自噬水平的影响，为OSCC的临床治疗提供理论基础。方法： 通过CCK8实验测定紫杉醇、ipatasertib在Cal-27细胞中单独用药及联合用药的吸光度，并比较相应的细胞致死率，获得最适宜的联合用药浓度；利用EDU实验观察紫杉醇、ipatasertib在单独用药及联合用药下的细胞增殖情况；通过细胞免疫荧光法比较不同组别间自噬相关蛋白LC3的表达情况；通过蛋白质印迹法检测自噬相关蛋白（mTOR、LC3、Beclin-1、P62）在不同组别中的表达情况。结果：紫杉醇、ipatasertib单独使用和联合用药均能够促进Cal-27细胞的死亡和增殖，且联合用药造成的细胞致死率和抑制效果高于单独用药；紫杉醇、ipatasertib单独使用及联合用药均增强了Cal-27细胞自噬，但联合用药作用效果强于单独用药。结论：紫杉醇、ipatasertib联合使用对OSCC细胞株Cal-27的死亡起到促进作用，对其增殖起到抑制作用，并且较单一用药效果增强，此外2种方式均不同程度地促进了自噬的发生。

关键词: 口腔鳞状细胞癌, 紫杉醇, ipatasertib, Cal-27细胞, 自噬

Abstract:

Objective: To investigate the effect of paclitaxel combined with ipatasrtib on the oral squamous cell carcinoma (OSCC) cell line Cal-27 to study the changes of the proliferation level and autophagy level, which will provide the basis for further research and clinical application of oral squamous cell carcinoma (OSCC).Methods: The optical density values ​​of paclitaxel and ipatasertib alone and in combination in Cal-27 cell line were determined by CCK8 assay, and the lethality of the corresponding drugs was further compared to find the most suitable concentration of combination therapy. The expression of autophagy-related protein LC3 among different groups was compared by cell immunofluorescence assay; the autophagy-related protein (mTOR, LC3, Beclin-1, P62) of different groups was observed by Western blotting.Results: Both paclitaxel and ipatasertib alone and in combination promoted the death and proliferation of Cal-27 cells, and the combination resulted in higher cell lethality and inhibition than alone; both paclitaxel and ipatasertib alone and in combination enhanced autophagy in Cal-27 cells, and the combination was more effective than alone.Conclusion: Paclitaxel combined with ipatasertib can promote the death of OSCC cell line Cal-27 and inhibit its proliferation, and the effect was enhanced compared with that of a single drug, and both methods promoted the occurrence of autophagy to varying degrees.

Key words: oral squamous cell carcinoma, paclitaxel, ipatasertib, Cal-27 cells, autophagy

中图分类号: 

• R782