兔骨髓干细胞和脂肪干细胞体外成骨能力的比较

doi:10.3969/j.issn.1005-4979.2013.01.002

《口腔颌面外科杂志》 ›› 2013, Vol. 23 ›› Issue (1): 8-13. doi: 10.3969/j.issn.1005-4979.2013.01.002

兔骨髓干细胞和脂肪干细胞体外成骨能力的比较

王之发，翁雁鸣，刘彦普，刘斌

第四军医大学口腔医院口腔颌面外科，陕西西安　710032

出版日期:2013-02-28 发布日期:2013-05-08
通讯作者: 刘彦普，教授. E-mail：liuyanpu@fmmu.edu.cn；刘斌，教授. E-mail：kqyljd@fmmu.edu.cn
作者简介:王之发（1987—），男，安徽亳州人，硕士研究生.E-mail：wangzf19871013@163.com
基金资助:
国家自然科学基金资助项目（81070820， 31170942）；陕西省13115科技创新工程重大项目（2009ZDK74）

Osteogenic Potential of Bone Marrow vs Adipose Tissue Mesenchymal Stem Cells

WANG Zhi-Fa，WENG Yan-ming， LIU Yan-pu， LIU Bin

Department of Oral and Maxillofacial Surgery，School of Stomatology, Fourth Military Medical University, Xi′an 710032, Shaanxi Province, China

Online:2013-02-28 Published:2013-05-08

摘要/Abstract

摘要： 目的： 评价两种不同来源的间充质干细胞，即骨髓干细胞（BMSC）和脂肪干细胞（ADSC）的体外成骨能力，为其将来应用于细胞治疗和组织工程研究提供一定的理论依据。方法：分别取同一只兔的髂骨骨髓和腹股沟脂肪作为BMSC和ADSC的来源，分离培养后比较成骨、成脂分化潜能，细胞表面抗原标记物；成骨诱导后检测碱性磷酸酶（ALP）活性，RT-PCR分析其成骨相关基因的表达情况。结果：BMSC和ADSC都具有向成骨、成脂分化的潜能；在成骨诱导分化后，BMSC有较高的ALP活性和较多的钙结节形成。RT-PCR结果显示：BMSC表达较高的BMP-2、OCN和OPN等成骨基因。结论： BMSC和ADSC都具有向成骨、成脂分化的潜能， ADSC有较好的成脂分化能力，BMSC有较好的成骨分化能力；两者都可以作为组织工程的种子细胞。

关键词: 干细胞, 　骨髓基质干细胞, 　脂肪干细胞, 　成骨分化, 　组织工程

Abstract: Objective: This study was designed to evaluate the osteogenic capacity of rabbit mesenchymal stem cells both isolated from bone marrow (BMSCs) and adipose tissue (ADSCs) in vitro. Methods: BMSCS and ADSCS were obtained from iliac bone marrow and inguinal fat tissue respectively of the same animal. BMSCs and ADSCs were cultured in the medium and their third-passage cells were used for experiments. We compared their multi-differentiation potential, and cell surface antigen markers. Alkaline phosphatase (ALP) activity was assayed as well as to assess gene expression by RT-PCR. Results: BMSCs and ADSCs both have multilineage differentiation potentials. BMSCs expressed highest ALP activity and more calcium nodule formation after osteogenic differentiation. RT-PCR results showed that BMSCs also express higher osteogenic genes like BMP-2, OCN and OPN. Conclusion: BMSCs and ADSCs both have multilineage differentiation potentials; but compared to the ADSCs which expressed more adipogenic differentiation capacity, the BMSCs osteogenic differentiation capacity is more better. Both of them can be used as seed cells for tissue engineering.

Key words: stem cell, bone marrow stem cell, adipose-deprived stem cell, osteogenic differentiation, tissue engineering

中图分类号:

R329.2

王之发，翁雁鸣，刘彦普，刘斌. 兔骨髓干细胞和脂肪干细胞体外成骨能力的比较[J]. 《口腔颌面外科杂志》, 2013, 23(1): 8-13.

WANG Zhi-Fa，WENG Yan-ming， LIU Yan-pu， LIU Bin. Osteogenic Potential of Bone Marrow vs Adipose Tissue Mesenchymal Stem Cells[J]. 《Journal of Oral and Maxillofacial Surgery》, 2013, 23(1): 8-13.

https://journal06.magtech.org.cn/Jweb_joms/CN/Y2013/V23/I1/8

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