摘要： 目的： 观察YAP2蛋白在涎腺腺样囊性癌组织及细胞中的表达。方法： 通过免疫组织化学法检测YAP2蛋白在涎腺腺样囊性癌组织及细胞中的表达，用Western印迹法检测表皮生长因子受体(epidermal growth factor receptor, EGFR)对涎腺腺样囊性癌（salivary gland adenoid cystic carcinoma，SACC）SACC-83细胞YAP2蛋白的抑制。结果：SACC组织中YAP2蛋白在肿瘤细胞胞质中阳性表达，正常腺体中YAP2蛋白主要表达在部分导管上皮中，两组相比差异有统计学意义（P＜0.05）。免疫细胞化学染色结果显示SACC-83中YAP2蛋白阳性表达，加入EGFR蛋白后，SACC-83中YAP2蛋白表达有所降低。Western印迹法结果显示，不同质量浓度的EGFR蛋白（5、10、20、40 μg/L）对SACC-83细胞YAP2蛋白的表达都有一定抑制作用，其中40 μg/L质量浓度组抑制作用最明显。结论：YAP2蛋白在涎腺腺样囊性癌组织及细胞中高表达，EGFR蛋白对YAP2蛋白在SACC-83中表达有抑制作用。
关键词:
YAP2； ,
涎腺腺样囊性癌； ,
SACC-83； ,
表皮生长因子受体
Abstract: Objective: This study aimed to observe the expression of YAP2 in salivary gland adenoid cystic carcinoma tissue and cell line. Methods: YAP2 protein in salivary gland adenoid cystic carcinoma tissue and cells were identified by immunohistochemistry with a multiclonal antibody. EGFR inhibition of YAP2 in SACC-83 cells was detected by western blotting. Results: In SACC, positive expression of YAP2 were in the cytoplasm. In normal salivary gland, YAP2 positive were identified in some duct epithelia. There was statistical significance between them (P<0.05). Expression of YAP2 protein in SACC-83 was down-regulated by the addition of EGFR. Western blot analysis showed EGFR（5, 10, 20, 40 μg/L）inhibited the expression of YAP2 in SACC-83, and 40 μg/L was most effective. Conclusion: YAP2 protein is overexpressed in salivary gland adenoid cystic carcinoma tissue and cells. EGFR protein inhibits the expression of YAP2 in the SACC-83.
Key words:
YAP2,
SACC,
SACC-83,
EGFR
中图分类号:
李莹1， 王喜华2， 于洋2， 刘培红2， 马肃2， 赵尔飏2. YAP2蛋白在涎腺腺样囊性癌组织及细胞中的表达[J]. 《口腔颌面外科杂志》, 2013, 23(5): 357-360.
LI Ying1，WANG Xi-hua2, YU Yang2, LIU Pei-hong2, MA Su2, ZHAO Er-yang2. Role of EGFR on Expression of YAP2 in Salivary Adenoid Cystic Carcinoma[J]. 《Journal of Oral and Maxillofacial Surgery》, 2013, 23(5): 357-360.