摘要： 目的：研究表没食子儿茶素没食子酸酯（epigallocatechin-3-gallate, EGCG）对舌鳞癌细胞CAL-27的表皮生长因子受体（epidermal growth factor receptor, EGFR）、相对分子质量为67 000的层粘连蛋白受体（67 000 dalton laminin receptor，67LR）及血管内皮生长因子（vascular endothelial growth factor receptor, VEGF）表达的影响，从而揭示EGCG抑致舌鳞癌细胞增殖的可能机制。方法：不同浓度EGCG处理CAL-27细胞24h后，MTT法检测细胞增殖活性；Real Time PCR法和Western Blot法分别检测CAL-27细胞EGFR、67LR、VEGF的mRNA及蛋白表达情况；Western Blot法检测磷酸化EGFR（p-EGFR）的蛋白表达水平。结果：EGCG浓度依赖性抑制CAL-27细胞的增殖，且抑制其EGFR、67LR、VEGF的mRNA和蛋白、p-EGFR的蛋白表达。结论：EGCG能影响EGFR、67LR及VEGF的转录和翻译水平以及p?鄄EGFR蛋白表达，这可能是EGCG抑制舌鳞癌细胞CAL-27增殖的重要机制。

关键词: 表没食子儿茶素没食子酸酯, 舌鳞癌细胞, 表皮生长因子受体, 层粘连蛋白受体, 血管内皮生长因子

Abstract: Objective: This study was designed to detect the effect of epigallocatechin-3-gallate (EGCG) on the expression of EGFR, 67LR and VEGF in human tongue squamous cell carcinoma cell line CAL-27, and the role of proliferation inhibition to cancer cells.  Methods: Human tongue squamous cell carcinoma cell line CAL-27 were treated with EGCG  for 24h. Cell viability was assessed by MTT assay. The mRNA levels of EGFR, 67LR and VEGF  were measured by real- time PCR and their protein levels were determined by Western Blot. The protein expression of p-EGFR were evaluated by Western Blot. Results: EGCG showed  inhibition effect on CAL-27 cells proliferation.  Suppression effect of the mRNA levels and protein levels of EGFR, 67LR and VEGF, protein levels of p-EGFR were manifested in a dose-dependent manner. Conclusion: EGCG inhibited the mRNA and protein expression of EGFR, 67LR and VEGF, as well as protein levels of p-EGFR, which may be associated with its suppression effect of proliferation of CAL-27 cells.

Key words: epigallocatechin-3-gallate, tongue squamous cell carcinoma cell, EGFR, 67 000 dalton laminin receptor(67LR), VEGF

中图分类号: 

• R739.86